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PMID: 3004956 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants.

The EMBO journal ·Vol. 4 ·No. 12 ·1985-12-01 ·Pages 3357-65

Murphy E, Huwyler L, de Freire Bastos Mdo C

Abstract

The complete nucleotide sequence of the Staphylococcus aureus transposon Tn554, which encodes resistance to erythromycin and spectinomycin, was determined by the dideoxy chain termination method. The transposon was found to be 6691 bp in length and to contain six open reading frames of greater than 125 amino acids. Small insertion and deletion mutations were obtained in each of these by in vitro mutagenesis at restriction endonuclease cleavage sites and the mutants characterized with respect to transposition functions and antibiotic resistance markers. Three of the reading frames, designated tnpA, tnpB and tnpC, encode functions that are required for transposition of Tn554; genetic analysis indicated that these three genes define distinct complementation groups of transposition-defective mutants. Two of the open reading frames correspond to the resistance determinants spc and ermA, the sixth, designated ORF, has no known function. Tn554-specific peptides corresponding to tnpA, and spc were identified in a coupled transcription-translation system in vitro.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Transposable Elements Drug Resistance, Microbial Erythromycin/pharmacology Mutation Phenotype Species Specificity Spectinomycin/pharmacology Staphylococcus aureus/drug effects,genetics
Chemicals
DNA Transposable Elements Erythromycin Spectinomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murphy E
Huwyler L
de Freire Bastos M do C
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52 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-01
Pages
3357-65
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554666
Subset
IM
Grants
NIGMS NIH HHS · GM27253 · United States
Databases
GENBANK
K02987
External Links
PubMed source PMC Full Text View journal details
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