Kinetics of Mu DNA synthesis.

Molecular & general genetics : MGG ·Vol. 151 ·No. 2 ·1977-03-07 ·Pages 169-74

Wijffelman C, Lotterman B

Abstract

Mu specific DNA synthesis starts at 10 min after infection. All essentail amber mutants of Mu were tested for the ability to replicate in a non permissive host. Except for the amber mutants A and B, which were already known to be blocked in Mu DNA synthesis (Wijffelman et al., 1974), all the other mutants showed normal Mu DNA replication. Using mitomycin C-treated cells Mu DNA synthesis was found to start at about 20 min after induction. However using the much more sensitive method of DNA-RNA hybridization, it was found that the DNA synthesis starts already at 10 min after induction, and that at 20 min after induction about 7 copies of the Mu DNA are present per cell.

MeSH Terms

Coliphages/drug effects,metabolism DNA, Viral/biosynthesis Kinetics Lysogeny Mitomycins/pharmacology Mutation Nucleic Acid Hybridization

Chemicals

DNA, Viral Mitomycins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wijffelman C

Lotterman B

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00338691

Published

1977-03-07

Pages

169-74

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00338691

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Kinetics of Mu DNA synthesis.

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