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PMID: 3923434 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translationally coupled initiation of protein synthesis in Bacillus subtilis.

Nucleic acids research ·Vol. 13 ·No. 3 ·1985-02-11 ·Pages 893-909

Sprengel R, Reiss B, Schaller H

Abstract

The neomycin phosphotransferase gene (neo) from Transposon Tn5 is active in Gram-negative bacteria but silent in B. subtilis since it lacks an appropriate ribosome binding site for Gram-positive bacteria. Neo translation could be reactivated by coupling its initiation to the translational termination of the highly expressed beta-lactamase gene (penP) from B. licheniformis. This initiation occurred at the authentic neo start codon. Its efficiency was independent of the nucleotide sequence 5 to the neo gene, but strongly affected by the distance between the termination and initiation codon. It was the highest if both codons overlapped in the sequence ATGA. In B. licheniformis, a translationally coupled neo gene was inducible expressed as the penP gene demonstrating the potential of the technique to monitor the activity of expression units for which no direct assays exists.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Gene Expression Regulation Plasmids Protein Biosynthesis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sprengel R
Reiss B
Schaller H
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.3.893
Published
1985-02-11
Pages
893-909
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341041
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.3.893 PMC Full Text View journal details
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