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PMID: 6269055 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.

Nucleic acids research ·Vol. 9 ·No. 11 ·1981-06-11 ·Pages 2577-88

Neugebauer K, Sprengel R, Schaller H

Abstract

The gene for the penicillinase from B. licheniformis has been cloned in a functional stat on a 1.5 kb DNA fragment and its nucleotide sequence has been determined. A sequence of 307 amino acid residues is infered for the penicillinase precursor. Of these 34 amino acids precede the sequence of the secreted form of the enzyme. This peptide extension shows the features of a signal for secretion and also provides the hydrophobic anchor for the membrane-bound form of the enzyme.

MeSH Terms
Bacillus/enzymology,genetics Bacteria/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Molecular Weight Penicillinase/genetics Species Specificity beta-Lactamases/genetics
Chemicals
DNA, Recombinant DNA Restriction Enzymes Penicillinase beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Neugebauer K
Sprengel R
Schaller H
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.11.2577
Published
1981-06-11
Pages
2577-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326873
Subset
IM
Databases
GENBANK
J01544, J01545
External Links
PubMed source DOI: 10.1093/nar/9.11.2577 PMC Full Text View journal details
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