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PMID: 6446019 Published · ppublish English Journal Article

Molecular cloning of the gene for the beta-lactamase of Bacillus licheniformis and its expression in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 178 ·No. 1 ·1980-04-00 ·Pages 217-24

Brammar WJ, Muir S, McMorris A

Abstract

The structural gene, pen, for the beta-lactamase of B. licheniformis has been cloned into a lambda vector and shown to be expressed at a low rate in E. coli. The cloned pen gene appears to be expressed from a promoter within the fragment of B. licheniformia DNA, since its rate of expression is not affected by the presence of the phage repressor, the absence of the phage's positive-control functions, or the position or orientation of the gene within the phage genome.

MeSH Terms
Bacillus/genetics Bacteriophage lambda/genetics Cloning, Molecular Escherichia coli/genetics Genes Penicillinase/genetics Phenotype Transformation, Bacterial beta-Lactamases/genetics
Chemicals
Penicillinase beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brammar W J
Muir S
McMorris A
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48 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267232
Published
1980-04-00
Pages
217-24
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267232
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