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PMID: 3038014 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.

Applied and environmental microbiology ·Vol. 53 ·No. 5 ·1987-05-00 ·Pages 1069-72

Yamamoto M, Jones JM, Senghas E, Gawron-Burke C, Clewell DB

Abstract

A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA containing the 16-kilobase conjugative transposon Tn916. It was possible to introduce some of the resulting Tn916::Tn5 derivatives back into S. faecalis by using a recently developed protoplast transformation procedure. A presumed zygotic induction resulted in insertion of the Tn916 derivatives at multiple sites in the S. faecalis chromosome.

MeSH Terms
Bacteriophage lambda Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/analysis,genetics Enterococcus faecalis/genetics Escherichia coli/genetics Nucleic Acid Hybridization Plasmids Protoplasts Transformation, Bacterial
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yamamoto M
Jones J M
Senghas E
Gawron-Burke C
Clewell D B
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20 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.5.1069-1072.1987
Published
1987-05-00
Pages
1069-72
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203811
Subset
IM
Grants
NIAID NIH HHS · AI10318 · United States
External Links
PubMed source DOI: 10.1128/aem.53.5.1069-1072.1987 PMC Full Text View journal details
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