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PMID: 6094500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.

Journal of bacteriology ·Vol. 160 ·No. 3 ·1984-12-00 ·Pages 1109-14

Smith MD, Clewell DB

Abstract

Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homologous sequences resolved the cointegrate in the S. faecalis transconjugants, and pVA797 and pVA838 segregated because of incompatibility. S. faecalis strains that received pVA838 by this mechanism contained plasmids indistinguishable from authentic pVA838 from Escherichia coli. Other plasmids, including pVA736, were introduced into S. faecalis by this method. This approach should facilitate the introduction of cloned DNA into S. faecalis.

MeSH Terms
Cloning, Molecular Conjugation, Genetic Crosses, Genetic DNA/metabolism DNA Restriction Enzymes DNA, Bacterial/genetics Enterococcus faecalis/genetics Escherichia coli/genetics Genetic Vectors Plasmids Transformation, Bacterial
Chemicals
DNA, Bacterial DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith M D
Clewell D B
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.3.1109-1114.1984
Published
1984-12-00
Pages
1109-14
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215827
Subset
IM
Grants
NIAID NIH HHS · AI10318 · United States
NIDCR NIH HHS · DE 02731 · United States
External Links
PubMed source DOI: 10.1128/jb.160.3.1109-1114.1984 PMC Full Text View journal details
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