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PMID: 6998966 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Improved method for conjugative transfer by filter mating of Streptococcus pneumoniae.

Journal of bacteriology ·Vol. 144 ·No. 1 ·1980-10-00 ·Pages 457-9

Smith MD, Guild WR

Abstract

The frequency of conjugation during filter mating of pneumococcus was increased 10- to 100-fold when the filter was embedded in agar during incubation instead of being on the surface. The major effect was not due to protection from oxygen. The factor of increase was similar for transfer of plasmids and of chromosomal insertions of drug resistance elements.

MeSH Terms
Agar Bacteriological Techniques Conjugation, Genetic Filtration/instrumentation Streptococcus pneumoniae/genetics
Chemicals
Agar
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith M D
Guild W R
References (5)
5 references, click to expand
  1. Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.
    J Bacteriol. 1976 Jan;125(1):125-35 PMID: 1370
  2. Characterization of some pneumococcal bacteriophages.
    J Virol. 1976 Aug;19(2):659-67 PMID: 8652
  3. Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus.
    J Bacteriol. 1979 Aug;139(2):432-41 PMID: 37238
  4. Transfer of plasmids by conjugation in Streptococcus pneumonias.
    Plasmid. 1980 Jan;3(1):70-9 PMID: 6278525
  5. DNase-resistant transfer of chromosomal cat and tet insertions by filter mating in Pneumococcus.
    Plasmid. 1980 Jan;3(1):80-7 PMID: 6278526
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.144.1.457-459.1980
Published
1980-10-00
Pages
457-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294685
Subset
IM
Grants
NIGMS NIH HHS · GM07754 · United States
NIGMS NIH HHS · GM21887 · United States
External Links
PubMed source DOI: 10.1128/jb.144.1.457-459.1980 PMC Full Text View journal details
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