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PMID: 3881405 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural organization of a 67-kilobase streptococcal conjugative element mediating multiple antibiotic resistance.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 620-6

Inamine JM, Burdett V

Abstract

The molecular organization of the conjugative cat-erm-tet region of Streptococcus agalactiae B109 was examined by cloning large contiguous portions of the strain B109 chromosome, using a cosmid vector system. The organization of this region was compared with pDP5, a plasmid which acquired this resistance element by transposition. Both the chromosomal copy and the transposed copy of the resistance region were found to be 67-kilobases long, although sequences at the boundary of the transposed copy of the element showed some rearrangement. In addition to the stable chromosomal state, we present evidence which suggests the presence of a circular form of the element.

MeSH Terms

Anti-Bacterial Agents/pharmacology Cloning, Molecular Conjugation, Genetic DNA, Bacterial/analysis Genetic Vectors Nucleic Acid Hybridization R Factors Streptococcus agalactiae/drug effects,genetics

Chemicals

Anti-Bacterial Agents DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Inamine J M

Burdett V

References (25)

25 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.161.2.620-626.1985

Published

1985-02-00

Pages

620-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214928

Subset

Grants

NIAID NIH HHS · AI01748 · United States

NIAID NIH HHS · AI15619 · United States

External Links

PubMed source DOI: 10.1128/jb.161.2.620-626.1985 PMC Full Text View journal details

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Structural organization of a 67-kilobase streptococcal conjugative element mediating multiple antibiotic resistance.

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