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PMID: 6330497 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translocation of antibiotic resistance markers of a plasmid-free Streptococcus pyogenes (group A) strain into different streptococcal hemolysin plasmids.

Molecular & general genetics : MGG ·Vol. 194 ·No. 3 ·1984-00-00 ·Pages 377-87

Le Bouguenec C, Horaud T, Bieth G, Colimon R, Dauguet C

Abstract

Wild-type strain A454 (Streptococcus pyogenes) transferred en bloc its erythromycin (Em) and tetracycline (Tc) resistance markers into several plasmid-free streptococcal recipients. No plasmid DNA was detected in either the wild-type or the transconjugant strains. Crosses were performed between A454 and S. faecalis Rec+ or Rec- recipients carrying hemolysin-bacteriocin plasmids, pIP964 or pAD1 . The Em Tc-resistant transconjugants obtained harbored either the parental plasmid or an Em Tc resistance plasmid derived from pIP964 or pAD1 . The restriction endonuclease analysis of 12 derivative plasmids showed insertions of various sizes into different fragments of pIP964 or pAD1 . A454 and the Em Tc-resistant plasmid-free transconjugants were found to contain two EcoRI DNA fragments, that shared homology with 32P-labeled pIP1077 , one of the Em Tc resistance derivative plasmids, but not with 32P-labeled pIP964 . No homology was detected between pIP1077 and the cellular DNA of the antibiotic-susceptible recipients.

MeSH Terms
Conjugation, Genetic DNA Restriction Enzymes DNA, Bacterial/genetics Drug Resistance, Microbial Erythromycin/pharmacology Hemolysin Factors Plasmids Streptococcus pyogenes/drug effects,genetics Tetracycline/pharmacology
Chemicals
DNA, Bacterial Erythromycin DNA Restriction Enzymes Tetracycline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Le Bouguenec C
Horaud T
Bieth G
Colimon R
Dauguet C
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425548
Published
1984-00-00
Pages
377-87
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425548
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