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PMID: 6285813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conjugative R plasmids in Streptococcus faecium (group D).

Antimicrobial agents and chemotherapy ·Vol. 21 ·No. 5 ·1982-05-00 ·Pages 698-705

Le Bouguenec C, Horodniceanu T

Abstract

Ten isolates of Streptococcus faecium were found to be resistant to penicillin, tetracycline, macrolides and related drugs, streptomycin, and kanamycin, and four strains were resistant to chloramphenicol. Six of these 10 strains transferred all their resistance markers (except penicillin) by conjugation at a low frequency (10(-7) to 10(-9)). Several plasmids of different molecular weights were found in each of the wild-type strains. In 5 of 11 transconjugant strains, R plasmids were detected which had molecular weights identical to those of the plasmids found in the corresponding donor strain. Each of the six other transconjugants harbored one plasmid with a size different from those found in the corresponding donor strain, suggesting the occurrence of molecular events during or after conjugative transfer. None of the five tetracycline-resistant transconjugants contained detectable satellite DNA, HindIII restriction enzyme fingerprints of S. faecium resistance plasmids were different from the HindIII patterns of macrolide, aminoglycoside, and tetracycline resistance plasmids from other strains of streptococci.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacteriocins/biosynthesis Conjugation, Genetic Culture Media DNA Restriction Enzymes/metabolism DNA, Bacterial/isolation & purification Drug Resistance, Microbial R Factors Streptococcus/genetics,metabolism
Chemicals
Anti-Bacterial Agents Bacteriocins Culture Media DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Le Bouguenec C
Horodniceanu T
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40 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.21.5.698
Published
1982-05-00
Pages
698-705
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC181996
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.21.5.698 PMC Full Text View journal details
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