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PMID: 6780509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Naturally occurring macrolide-lincosamide-streptogramin B resistance in Bacillus licheniformis.

Journal of bacteriology ·Vol. 145 ·No. 1 ·1981-01-00 ·Pages 129-37

Docherty A, Grandi G, Grandi R, Gryczan TJ, Shivakumar AG, Dubnau D

Abstract

Resistance to the macrolide-lincosamide-streptogramin B (MLS) group of antibiotics is widespread and of clinical importance. B. Weisblum and his coworkers have demonstrated that this resistance is associated with methylation of the 23S ribosomal ribonucleic acid of the large ribosomal subunit which results in a diminished affinity of this organelle for these antibiotics (Lai et al, J. Mol. Biol. 74:67-72, 1973). We report that 10 of 15 natural isolates of Bacillus licheniformis, a common soil organism, are resistant to the MLS antibiotics. The properties of this resistance (high level of tolerance for erythromycin, broad cross-resistance spectrum, and inducibility) suggest that resistance is conferred as described above. The resistance determinant from one of these strains was cloned onto a B. subtilis plasmid vector, and the resulting hybrid plasmid (pBD90) was used to prepare radioactive probe deoxyribonucleic acid for hybridization studies. All of the resistance B. licheniformis strains studied exhibited homology with the pBD90 insert. Plasmid pBD90 showed no homology to the following staphylococcal and streptococcal MLS-resistance plasmids: pE194, pE5, pAM77, pI258. Plasmids pE194 and pE5, on the other hand, carry homologous MLS genes but showed no detectable homology to one another in their replication genes. pBD90 specified a 35,000-dalton erythromycin-inducible protein, detectable in minicells, which therefore appears different from the 29,000-dalton inducible resistance protein specified by pE194. We conclude that there are at least three distinct MLS resistance determinants to be found among gram-positive bacteria.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus/drug effects,genetics Bacterial Proteins/biosynthesis Cloning, Molecular Drug Resistance, Microbial Erythromycin/pharmacology Genes Nucleic Acid Hybridization Plasmids Virginiamycin/pharmacology
Chemicals
Anti-Bacterial Agents Bacterial Proteins Virginiamycin Erythromycin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Docherty A
Grandi G
Grandi R
Gryczan T J
Shivakumar A G
Dubnau D
References (39)
39 references, click to expand
  1. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies.
    Proc Natl Acad Sci U S A. 1965 Aug;54(2):491-8 PMID: 4956287
  2. Factors affecting transformation of Bacillus licheniformis.
    J Bacteriol. 1966 Mar;91(3):1012-20 PMID: 5929742
  3. The genetics of Bacillus licheniformis penicillinase: a preliminary analysis from studies on mutation and inter-strain and intra-strain transformations.
    J Gen Microbiol. 1965 Oct;41(1):7-21 PMID: 5883699
  4. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus.
    J Bacteriol. 1968 Apr;95(4):1335-42 PMID: 5646621
  5. Genetic and base sequence homologies in bacillus.
    Genetics. 1969 Jul;62(3):697-710 PMID: 4989093
  6. Altered methylation of ribosomal RNA in an erythromycin-resistant strain of Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):856-60 PMID: 5279527
  7. Erythromycin-inducible resistance in Staphylococcus aureus: requirements for induction.
    J Bacteriol. 1971 Jun;106(3):835-47 PMID: 4397638
  8. Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus".
    Ann Inst Pasteur (Paris). 1972 Dec;123(6):755-9 PMID: 4347774
  9. Minicells of Bacillus subtilis.
    J Bacteriol. 1973 May;114(2):860-73 PMID: 4196259
  10. Alteration of 23 S ribosomal RNA and erythromycin-induced resistance to lincomycin and spiramycin in Staphylococcus aureus.
    J Mol Biol. 1973 Feb 15;74(1):67-72 PMID: 4731016
  11. A variety of Staphylococcal plasmids present as multiple copies.
    J Gen Microbiol. 1973 Dec;79(2):343-5 PMID: 4772094
  12. Erythromycin resistant mutants of Bacillus subtilis.
    Mol Gen Genet. 1973 Dec 20;127(2):157-61 PMID: 4203931
  13. Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins.
    Mol Gen Genet. 1973 Dec 20;127(2):175-89 PMID: 4589347
  14. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  15. Characterization of a plasmid determining resistance to erythromycin, lincomycin, and vernamycin Balpha in a strain Streptococcus pyogenes.
    Antimicrob Agents Chemother. 1974 May;5(5):534-7 PMID: 4618462
  16. Mutant of Staphylococcus aureus with lincomycin- and carbomycin-inducible resistance to erythromycin.
    Antimicrob Agents Chemother. 1974 May;5(5):538-40 PMID: 4462467
  17. R plasmids in Streptococcus agalactiae (group B).
    Antimicrob Agents Chemother. 1976 Nov;10(5):795-801 PMID: 795369
  18. Three distinct plasmids originating in the same Staphylococcus aureus strain.
    Arch Roum Pathol Exp Microbiol. 1976 Jan-Jun;35(1-2):111-8 PMID: 1008685
  19. Macrolide resistance in Staphylococcus aureus: inducers of macrolide resistance.
    Antimicrob Agents Chemother. 1977 Apr;11(4):669-74 PMID: 856018
  20. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  21. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.
    J Mol Biol. 1977 Jul;114(1):153-68 PMID: 20509
  22. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.
    J Bacteriol. 1978 Apr;134(1):318-29 PMID: 418061
  23. Characterization of a small plasmid determining resistance to erythromycin, lincomycin, and vernamycin Balpha in a strain of Streptococcus sanguis isolated from dental plaque.
    Antimicrob Agents Chemother. 1978 May;13(5):884-7 PMID: 96738
  24. Emergence of multiply resistant pneumococci.
    N Engl J Med. 1978 Oct 5;299(14):735-40 PMID: 29219
  25. A specific endonuclease from Bacillus caldolyticus.
    Nucleic Acids Res. 1978 Oct;5(10):3457-67 PMID: 724493
  26. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194.
    J Bacteriol. 1979 Jan;137(1):635-43 PMID: 104975
  27. The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis.
    Mol Gen Genet. 1978 Nov 9;166(3):259-67 PMID: 105241
  28. Site specific endonuclease from Fusobacterium nucleatum.
    Nucleic Acids Res. 1979 Jan;6(1):1-15 PMID: 424284
  29. Genetic translocation in Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):400-4 PMID: 284355
  30. 23S ribosomal ribonucleic acid of macrolide-producing streptomycetes contains methylated adenine.
    J Bacteriol. 1979 Mar;137(3):1464-7 PMID: 438126
  31. Transferable lincosamide-macrolide resistance in Bacteroides.
    Plasmid. 1979 Apr;2(2):261-8 PMID: 451051
  32. Studies on the synthesis of plasmid-coded proteins and their control in Bacillus subtilis minicells.
    Plasmid. 1979 Apr;2(2):279-89 PMID: 109873
  33. Deoxyribonucleic acid sequence common to staphylococcal and streptococcal plasmids which specify erythromycin resistance.
    J Bacteriol. 1979 Jun;138(3):990-8 PMID: 110774
  34. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis.
    J Bacteriol. 1980 Jan;141(1):246-53 PMID: 6243622
  35. Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis.
    J Bacteriol. 1980 Mar;141(3):1178-82 PMID: 6245061
  36. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
    Methods Enzymol. 1979;68:220-42 PMID: 94421
  37. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  38. Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid in Bacillus subtilis.
    Mol Gen Genet. 1980 Feb;177(3):459-67 PMID: 6246400
  39. Organization of the pE194 genome.
    Mol Gen Genet. 1980;179(2):241-52 PMID: 6258012
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.1.129-137.1981
Published
1981-01-00
Pages
129-37
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217253
Subset
IM
Grants
NIAID NIH HHS · AI-10311 · United States
External Links
PubMed source DOI: 10.1128/jb.145.1.129-137.1981 PMC Full Text View journal details
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