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PMID: 105241 Published · ppublish English Journal Article

The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis.

Molecular & general genetics : MGG ·Vol. 166 ·No. 3 ·1978-11-09 ·Pages 259-67

Canosi U, Morelli G, Trautner TA

Abstract

DNA preparations of the chloramphenicol resistance determining S. aureus plasmids pC194, pC223, and PUB112 can be fractionated by gel electrophoresis into various bands. Electronmicroscopic investigations of these various molecular species obtained with pC194 indicated that, depending on the preparations, 70 to 80% of the molecules were monomers, while the rest consisted of various classes of concatemeric and/or interlocked multimers. Measurements of the specific transforming activity of the various molecular classes indicated that the monomers had less than one thousandth the activity of the multimeric plasmic DNA. pC194 DNA of high specific transforming activity could also be obtained by ligation of HindIII generated monomers into concatemeric DNA.

MeSH Terms
Bacillus subtilis/genetics Chloramphenicol/pharmacology DNA, Bacterial Electrophoresis, Agar Gel Nucleic Acid Conformation R Factors Staphylococcus aureus/genetics Transformation, Bacterial
Chemicals
DNA, Bacterial Chloramphenicol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Canosi U
Morelli G
Trautner T A
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267617
Published
1978-11-09
Pages
259-67
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267617
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