Site specific endonuclease from Fusobacterium nucleatum.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 1-15

Lui AC, McBride BC, Vovis GF, Smith M

Abstract

Four different isolates of Fusobacterium nucleatum (A,C,D and E) contain restriction endonucleases of differing specificity. Whilst many of the endonucleases are isochizomers of known enzymes, two novel activities are Fnu DII which recognizes and cleaves the sequence 5'-CGCT-3'/3'-GCGC-5' AND Fnu EI which recognizes and cleaves the sequence 5'-GATC-3'/3'-CTAG-5' irrespective of the extent of methylation of the adenine residues.

MeSH Terms

Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism Fusobacterium/enzymology Molecular Weight Oligodeoxyribonucleotides/analysis Substrate Specificity

Chemicals

Oligodeoxyribonucleotides DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lui A C

McBride B C

Vovis G F

Smith M

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.1.1

Published

1979-01-00

Pages

1-15

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327669

Subset

External Links

PubMed source DOI: 10.1093/nar/6.1.1 PMC Full Text View journal details

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Site specific endonuclease from Fusobacterium nucleatum.

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