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PMID: 1824713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Positive regulation of jun/AP-1 by E1A.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 192-201

de Groot R, Foulkes N, Mulder M, Kruijer W, Sassone-Corsi P

Abstract

Proteins encoded by the adenovirus E1A oncogene are capable of positive and negative transcriptional regulation of both viral and cellular genes. E1A regulatory function is commonly thought to involve modifications of specific cellular factors that interact with responsive promoters. In this report we present evidence that E1A induces the activity of the jun/AP-1 transcription factor in three different cell types: P19, JEG-3, and HeLa. AP-1 binds to 12-O-tetradecanoylphorbol-13-acetate (TPA)-responsive elements (TREs); therefore, E1A might modulate a specific signal transduction pathway normally induced by activation of the protein kinase C. Binding of jun/AP-1 to a TRE is induced in all cell types studied when E1A is expressed. We observe that the expression of endogenous c-jun and jun B genes is induced by E1A, which directly transactivates the promoters of c-fos, c-jun, and jun B. Similar inducibility is obtained by treatment with retinoic acid and differentiation of P19-embryonal carcinoma cells. The E1A 13S product transactivates TRE sequences and cooperates with c-jun in the transcriptional stimulation. The 12S E1A product does not activate a TRE sequence, but cotransfection with c-jun circumvents this lack of stimulation. Coexpression of c-fos and E1A 12S, however, blocks the transactivation by c-jun, suggesting an important role for fos in determining the dominance of the 12S or 13S protein.

MeSH Terms
Adenovirus Early Proteins Cell Differentiation Cell Transformation, Viral DNA-Binding Proteins/physiology Gene Expression Regulation Humans In Vitro Techniques Oncogene Proteins, Viral/physiology Promoter Regions, Genetic Protein Binding Proto-Oncogene Proteins/physiology Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Regulatory Sequences, Nucleic Acid Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/physiology Transcription, Genetic Tretinoin/pharmacology Tumor Cells, Cultured
Chemicals
Adenovirus Early Proteins DNA-Binding Proteins Oncogene Proteins, Viral Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors Tretinoin Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
de Groot R
Hubrecht Laboratorium, Netherlands Institute for Developmental Biology, Utrecht.
Foulkes N
Mulder M
Kruijer W
Sassone-Corsi P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.192-201.1991
Published
1991-01-00
Pages
192-201
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359609
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.1.192-201.1991 PMC Full Text View journal details
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