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PMID: 6292525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA.

Journal of virology ·Vol. 44 ·No. 2 ·1982-11-00 ·Pages 674-82

Curran T, Peters G, Van Beveren C, Teich NM, Verma IM

Abstract

A 12.0-kilobase EcoRI restriction fragment containing FBJ murine osteosarcoma virus (FBJ-MSV) proviral DNA was identified in FBJ-MSV-transformed nonproducer rat cells and molecularly cloned in bacteriophage Charon 30 (lambda FBJ-1). A 5.8-kb HindIII fragment containing the entire FBJ-MSV proviral DNA was isolated from lambda FBJ-1 and subsequently subcloned in plasmid pBR322 (pFBJ-2). The DNA from recombinant plasmid pFBJ-2 was able to induce morphological transformation of rat fibroblasts in tissue culture. Transfected cells contained the p55 and p39 antigens specific for cells transformed by FBJ-MSV (T. Curran and N. M. Teich, J. Virol. 42:114-122, 1982). The organization of the FBJ-MSV provirus was analyzed by restriction endonuclease mapping, and a region of nonhomology with the helper virus was delineated. Sequences specific for this region (presumably the viral fos gene) were subcloned and used as a probe to identify related sequences present in the normal genomes of cells from a variety of mammalian species (cellular fos). A single-size (3.4 kilobases long) class of RNA hybridizing to the viral fos probe was identified in FBJ-MSV-transformed cells.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Cloning, Molecular Genes, Viral Mice Oncogenes RNA, Viral/genetics Rats Sarcoma Viruses, Murine/genetics Transcription, Genetic
Chemicals
RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Curran T
Peters G
Van Beveren C
Teich N M
Verma I M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.2.674-682.1982
Published
1982-11-00
Pages
674-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256311
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.44.2.674-682.1982 PMC Full Text View journal details
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