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PMID: 6248858 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2651-5

Jones M, Bosselman RA, van der Hoorn FA, Berns A, Fan H, Verma IM

Abstract

When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRI, a DNA fragment of 14.0 kilobases can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kilobase EcoRI DNA fragment was cloned in bacteriophage lambda. The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy. The results indicate that lambda . MTX-1 contains an uninterrupted stretch of 1.0 kilobase similar to that found in the M-MSV genome.

MeSH Terms
Animals Bacteriophage lambda/genetics Cloning, Molecular/methods DNA/genetics DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Mice/genetics,microbiology Microscopy, Electron Molecular Weight Moloney murine leukemia virus/genetics Nucleic Acid Hybridization
Chemicals
DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jones M
Bosselman R A
van der Hoorn F A
Berns A
Fan H
Verma I M
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2651
Published
1980-05-00
Pages
2651-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349460
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.2651 PMC Full Text View journal details
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