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PMID: 392518 Published · ppublish English Journal Article

Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6314-8

Tronick SR, Robbins KC, Canaani E, Devare SG, Andersen PR, Aaronson SA

Abstract

The unintegrated circular DNA form of Moloney murine sarcoma virus (MSV) has been cloned in bacteriophage lambda. Discrete deletions in the viral genome were shown to occur during propagation of recombinant phage in Escherichia coli. Heteroduplex and restriction enzyme analyses indicated the deletion of tandemly repeated sequences within certain of the cloned MSV DNA inserts. Cloned MSV DNA was used to prepare a probe composed of its acquired cellular (src) sequences, shown previously to be necessary for MSV transformation. Analysis of EcoRI digests of normal mouse cellular DNA revealed the presence of a single 14-kilobase-pair fragment containing these sequences which lacked contiguity with endogenous type C helper viral information of the same cells. Thus, the sarcoma virus-specific sequences of MSV are represented within the normal mouse genome in a manner analogous to that of a cellular gene.

MeSH Terms
Animals Chromosome Deletion Cloning, Molecular DNA/genetics DNA, Recombinant DNA, Viral/genetics Escherichia coli/genetics Gene Amplification Genes, Viral Helper Viruses/genetics Mice/genetics Moloney murine leukemia virus/genetics Recombination, Genetic Sarcoma, Experimental/genetics
Chemicals
DNA, Recombinant DNA, Viral DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tronick S R
Robbins K C
Canaani E
Devare S G
Andersen P R
Aaronson S A
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.12.6314
Published
1979-12-00
Pages
6314-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411854
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.12.6314 PMC Full Text View journal details
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