Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.

Journal of virology ·Vol. 4 ·No. 5 ·1969-11-00 ·Pages 549-53

Jainchill JL, Aaronson SA, Todaro GJ

Abstract

A continuous cell line of highly contact-inhibited cells (NIH/3T3) has been developed from NIH Swiss mouse embryo cultures. Its growth properties are similar to those of 3T3 and BALB/3T3. Although 3T3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both NIH/3T3 and BALB/3T3 have been isolated that are highly sensitive to sarcoma virus focus formation and leukemia virus growth. The sensitivity and specificity are comparable to those found with primary embryo cells. MSV-transformed lines of NIH/3T3 have been obtained.

MeSH Terms

Animals Cell Line Cell Transformation, Neoplastic Clone Cells Contact Inhibition Culture Techniques Embryo, Mammalian Fibroblasts Gammaretrovirus/isolation & purification Leukemia Virus, Murine/isolation & purification Mice

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jainchill J L

Aaronson S A

Todaro G J

References (13)

13 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.4.5.549-553.1969

Published

1969-11-00

Pages

549-53

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC375908

Subset

External Links

PubMed source DOI: 10.1128/JVI.4.5.549-553.1969 PMC Full Text View journal details

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Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.

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