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PMID: 2997786 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of the proto-oncogene fos by nerve growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 21 ·1985-11-00 ·Pages 7330-4

Kruijer W, Schubert D, Verma IM

Abstract

Nerve growth factor (NGF) causes the differentiation of PC12 cells to sympathetic neuron-like cells and also induces a rapid but transient expression of fos mRNA and protein. fos mRNA transcripts can be detected 5 min after the addition of NGF, are maximally abundant after 30 min, and then their levels decrease. fos protein synthesis parallels the expression of fos mRNA, and the induced fos proteins are located in the nucleus. cAMP, epidermal growth factor, the phorbol ester phorbol 12-myristate 13-acetate, and K+ depolarization also induce the fos gene. Growth of PC12 cells in the presence of dexamethasone, which induces differentiation into chromaffin-like cells, is not accompanied by fos expression. We propose that while fos gene induction is associated with the differentiation of PC12 cells to sympathetic nerve, its enhanced expression is primarily involved in the anabolic responses induced by NGF and many growth factors.

MeSH Terms
Animals Bucladesine/pharmacology Cell Differentiation/drug effects Cell Line Dexamethasone/pharmacology Epidermal Growth Factor/pharmacology Gene Expression Regulation/drug effects Neoplasm Proteins/biosynthesis Nerve Growth Factors/pharmacology Pheochromocytoma/genetics,pathology Potassium/pharmacology Proto-Oncogene Proteins/biosynthesis,genetics Proto-Oncogene Proteins c-fos Proto-Oncogenes RNA, Messenger/analysis RNA, Neoplasm/analysis Rats Tetradecanoylphorbol Acetate/pharmacology Transcriptional Activation
Chemicals
Neoplasm Proteins Nerve Growth Factors Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos RNA, Messenger RNA, Neoplasm Epidermal Growth Factor Bucladesine Dexamethasone Tetradecanoylphorbol Acetate Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kruijer W
Schubert D
Verma I M
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49 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.21.7330
Published
1985-11-00
Pages
7330-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391338
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.21.7330 PMC Full Text View journal details
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