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PMID: 2531661 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

TPA can overcome the requirement for EIa and together act synergistically in stimulating expression of the adenovirus EIII promoter.

The EMBO journal ·Vol. 8 ·No. 13 ·1989-12-20 ·Pages 4239-50

Buckbinder L, Miralles VJ, Reinberg D

Abstract

We have examined the control of gene expression from the adenovirus early region III (Ad-EIII) promoter, which contains two previously defined elements, the AP1 and ATF sites. We found that the AP1 element is capable of mediating activation by the adenovirus immediate early (EIa) gene products. Consistent with studies demonstrating that the AP1 site mediates signal transduction in response to 12-O-tetradecanoylphorbol 13-acetate (TPA) we have shown that TPA can activate Ad-EIII expression and overcome the requirement for EIa. Together TPA and EIa elicited a synergistic response in expression from the Ad-EIII promoter during both transient expression assays and viral infections. This synergistic effect required the AP1 element. An EIII promoter construct, in which sequences upstream of the TATA box had been replaced with four AP1 sites, was responsive to TPA and EIa and in combination promoted the synergistic effect. The analysis of specific factors involved in transcription from the Ad-EIII indicated that proteins recognizing the ATF and AP1 sites were important in expression from this promoter in vitro. Purification of protein factors that specifically stimulated EIII expression resulted in the isolation of a set of factors of the AP1 family. Affinity purified AP1 recognized and activated transcription through both the AP1 and ATF elements. In addition, a protein fraction was identified with DNA binding activity specific for the ATF element. This fraction was dependent on the ATF site for transcriptional activity.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Base Sequence Chromosome Deletion DNA-Binding Proteins/genetics Gene Expression Regulation, Viral/drug effects Genes, Viral HeLa Cells/metabolism Molecular Sequence Data Mutation Oligonucleotide Probes Oncogene Proteins, Viral/genetics Plasmids Promoter Regions, Genetic/drug effects Tetradecanoylphorbol Acetate/metabolism Transcription, Genetic Transfection Viral Structural Proteins/genetics
Chemicals
Adenovirus Early Proteins DNA-Binding Proteins Oligonucleotide Probes Oncogene Proteins, Viral Viral Structural Proteins Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buckbinder L
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Miralles V J
Reinberg D
References (66)
66 references, click to expand
  1. Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains.
    Science. 1989 Mar 31;243(4899):1695-9 PMID: 2494702
  2. Promoter occlusion prevents transcription of adenovirus polypeptide IX mRNA until after DNA replication.
    Genes Dev. 1989 Jan;3(1):49-59 PMID: 2523329
  3. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  4. An adenovirus E1a protein region required for transformation and transcriptional repression.
    Cell. 1986 Sep 26;46(7):1043-51 PMID: 2944600
  5. Adenovirus promoters and E1A transactivation.
    Annu Rev Genet. 1986;20:45-79 PMID: 3028247
  6. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE.
    J Biol Chem. 1987 Mar 5;262(7):3310-21 PMID: 3029109
  7. Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.
    J Biol Chem. 1987 Mar 5;262(7):3322-30 PMID: 3818643
  8. The adenovirus inverted terminal repeat functions as an enhancer in a cell-free system.
    J Biol Chem. 1989 Jun 25;264(18):10763-72 PMID: 2525131
  9. DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.
    Genes Dev. 1989 May;3(5):620-7 PMID: 2545525
  10. Accumulation of early and intermediate mRNA species during subgroup C adenovirus productive infections.
    Virology. 1984 Jul 30;136(2):387-403 PMID: 6205505
  11. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  12. Adenovirus E1a proteins repress transcription from the SV40 early promoter.
    Cell. 1985 Mar;40(3):705-16 PMID: 2982503
  13. Transcriptional analysis of the adenovirus-5 EIII promoter: absence of sequence specificity for stimulation by EIa gene products.
    Nucleic Acids Res. 1985 Feb 25;13(4):1209-21 PMID: 4000937
  14. Enhancement of RNA polymerase III transcription by the E1A gene product of adenovirus.
    Cell. 1985 Jul;41(3):955-63 PMID: 4005953
  15. Adenovirus E3-early promoter: sequences required for activation by E1A.
    Nucleic Acids Res. 1985 Jul 25;13(14):5389-402 PMID: 2991862
  16. Transcription of class III genes activated by viral immediate early proteins.
    Science. 1985 Oct 25;230(4724):447-50 PMID: 2996135
  17. Identification of a cellular transcription factor involved in E1A trans-activation.
    Cell. 1986 Apr 25;45(2):219-28 PMID: 2938741
  18. Adenovirus stimulation of transcription by RNA polymerase III: evidence for an E1A-dependent increase in transcription factor IIIC concentration.
    EMBO J. 1986 Feb;5(2):343-54 PMID: 2940084
  19. E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.
    Mol Cell Biol. 1985 Oct;5(10):2653-61 PMID: 2942760
  20. Affinity purification of sequence-specific DNA binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5889-93 PMID: 3461465
  21. A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells.
    Anal Biochem. 1986 Jul;156(1):251-6 PMID: 3461723
  22. Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2180-4 PMID: 2951737
  23. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  24. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  25. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene.
    Nature. 1987 Jul 9-15;328(6126):175-8 PMID: 2885756
  26. A cellular transcription factor E4F1 interacts with an E1a-inducible enhancer and mediates constitutive enhancer function in vitro.
    EMBO J. 1987 May;6(5):1345-53 PMID: 2956091
  27. Differential splicing yields novel adenovirus 5 E1A mRNAs that encode 30 kd and 35 kd proteins.
    EMBO J. 1987 Jul;6(7):2027-35 PMID: 2958276
  28. Multiple cis- and trans-acting elements mediate the transcriptional response to phorbol esters.
    Nature. 1987 Oct 15-21;329(6140):648-51 PMID: 2821407
  29. Upstream regulatory regions required to stabilize binding to the TATA sequence in an adenovirus early promoter.
    Nucleic Acids Res. 1987 Oct 26;15(20):8367-85 PMID: 2959908
  30. A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8355-9 PMID: 2960975
  31. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  32. Identification of factors that interact with the E1A-inducible adenovirus E3 promoter.
    Genes Dev. 1987 Dec;1(10):1132-46 PMID: 2828166
  33. Activation of a preexisting cellular factor as a basis for adenovirus E1A-mediated transcription control.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):387-90 PMID: 2963331
  34. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.
    Nature. 1988 Mar 10;332(6160):166-71 PMID: 3347253
  35. EIa-mediated stimulation of the adenovirus EIII promoter involves an enhancer element within the nearby EIIa promoter.
    J Virol. 1988 May;62(5):1762-7 PMID: 2965764
  36. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.
    Proc Natl Acad Sci U S A. 1988 May;85(10):3396-400 PMID: 2835770
  37. Fos-associated protein p39 is the product of the jun proto-oncogene.
    Science. 1988 May 20;240(4855):1010-6 PMID: 3130660
  38. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.
    Genes Dev. 1988 Mar;2(3):267-81 PMID: 3288540
  39. Activation of transcription factor IIIC by the adenovirus E1A protein.
    Cell. 1988 Jun 17;53(6):907-20 PMID: 2968157
  40. An adenovirus E1A protein domain activates transcription in vivo and in vitro in the absence of protein synthesis.
    Cell. 1988 Jun 17;53(6):921-6 PMID: 2968158
  41. Factors involved in specific transcription by mammalian RNA polymerase II. RNA polymerase II-associating protein 30 is an essential component of transcription factor IIF.
    J Biol Chem. 1988 Aug 5;263(22):10812-6 PMID: 3392044
  42. Cellular transcription factor binds to adenovirus early region promoters and to a cyclic AMP response element.
    J Virol. 1988 Aug;62(8):3003-13 PMID: 2839717
  43. DNA-binding properties of an adenovirus 289R E1A protein.
    EMBO J. 1988 Mar;7(3):835-41 PMID: 2969333
  44. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
    Cell. 1977 May;11(1):223-32 PMID: 194704
  45. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
    Cell. 1979 Jul;17(3):683-9 PMID: 476833
  46. An adenovirus type 5 early gene function regulates expression of other early viral genes.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3665-9 PMID: 291030
  47. Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site.
    Nature. 1982 Feb 4;295(5848):380-4 PMID: 7057903
  48. Mechanism of activation of early viral transcription by the adenovirus E1A gene product.
    Cell. 1981 Oct;26(2 Pt 2):213-20 PMID: 7332929
  49. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  50. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  51. Identification of adenovirus genes that require template replication for expression.
    J Virol. 1983 Jun;46(3):737-48 PMID: 6854739
  52. E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.
    Mol Cell Biol. 1983 Jul;3(7):1222-34 PMID: 6888379
  53. Complete transformation by adenovirus 2 requires both E1A proteins.
    Cell. 1984 Apr;36(4):951-61 PMID: 6705049
  54. Role of the adenovirus E1B 19,000-dalton tumor antigen in regulating early gene expression.
    J Virol. 1988 Sep;62(9):3445-54 PMID: 2969984
  55. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.
    Nature. 1988 Aug 11;334(6182):494-8 PMID: 2900470
  56. Induction of proto-oncogene JUN/AP-1 by serum and TPA.
    Nature. 1988 Aug 18;334(6183):629-31 PMID: 2457172
  57. EivF, a factor required for transcription of the adenovirus EIV promoter, binds to an element involved in EIa-dependent activation and cAMP induction.
    Genes Dev. 1988 Aug;2(8):975-90 PMID: 2844626
  58. Cyclic AMP induction of early adenovirus promoters involves sequences required for E1A trans-activation.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7192-6 PMID: 2902626
  59. Induction of protooncogene c-jun by serum growth factors.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8464-7 PMID: 3186736
  60. The jun proto-oncogene is positively autoregulated by its product, Jun/AP-1.
    Cell. 1988 Dec 2;55(5):875-85 PMID: 3142689
  61. c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinities.
    Cell. 1988 Dec 2;55(5):917-24 PMID: 3142692
  62. The role of the leucine zipper in the fos-jun interaction.
    Nature. 1988 Dec 15;336(6200):646-51 PMID: 2974122
  63. Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain.
    Nature. 1988 Dec 15;336(6200):692-5 PMID: 3143919
  64. A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1.
    Genes Dev. 1988 Oct;2(10):1216-26 PMID: 3144478
  65. cAMP acts in synergy with E1A protein to activate transcription of the adenovirus early genes E4 and E1A.
    Genes Dev. 1988 Dec;2(12A):1517-28 PMID: 2850966
  66. Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
    Science. 1989 Mar 31;243(4899):1689-94 PMID: 2494701
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-20
Pages
4239-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401624
Subset
IM
Grants
NIGMS NIH HHS · GM 37120 · United States
External Links
PubMed source PMC Full Text View journal details
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