Home LiteratureArticle Details

PMID: 2900470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.

Nature ·Vol. 334 ·No. 6182 ·1988-08-11 ·Pages 494-8

Yamamoto KK, Gonzalez GA, Biggs WH, Montminy MR

Abstract

A nuclear protein, CREB, has been isolated from rat brain and shown to stimulate transcription of the cyclic AMP-responsive gene somatostatin as a dimer. Biochemical analysis suggests that dimerization and transcriptional efficacy of CREB protein in vitro are regulated by phosphorylation. These findings demonstrate that cellular signals can modulate gene expression by regulating the covalent modification of pre-existing nuclear factors.

MeSH Terms

Acetyltransferases/genetics Animals Brain Chemistry Chloramphenicol O-Acetyltransferase Chromatography Cyclic AMP/pharmacology DNA/metabolism Electrophoresis, Polyacrylamide Gel Macromolecular Substances Molecular Weight Nuclear Proteins/metabolism Phosphorylation Protein Kinase C/metabolism Protein Kinases/metabolism Rats Repetitive Sequences, Nucleic Acid Somatostatin/genetics Transcription, Genetic Transfection Tumor Cells, Cultured

Chemicals

Macromolecular Substances Nuclear Proteins Somatostatin DNA Cyclic AMP Acetyltransferases Chloramphenicol O-Acetyltransferase Protein Kinases Protein Kinase C

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yamamoto K K

Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.

Gonzalez G A

Biggs W H

Montminy M R

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/334494a0

Published

1988-08-11

Pages

494-8

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/334494a0 View journal details

Analysis Services

Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.

Contact

WeChat Official Account