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PMID: 6854739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of adenovirus genes that require template replication for expression.

Journal of virology ·Vol. 46 ·No. 3 ·1983-06-00 ·Pages 737-48

Crossland LD, Raskas HJ

Abstract

The relationship between adenovirus type 2 DNA replication and expression of intermediate stage viral genes was investigated. The 1.03-kilobase mRNA from early region 1b (E1b) and the mRNAs coding for proteins IX and IVa2 were first detected between 6 and 8 h postinfection. Inhibition of viral DNA replication with hydroxyurea prevented expression of the IX and IVa2 mRNAs, but not of the E1b mRNA. Pulse-labeling experiments demonstrated that the block of IX and IVa2 expression in hydroxyurea-treated cells was at the level of transcription. By a series of superinfection experiments, it was determined that the viral and cellular factors present during the late stage of adenovirus infection are insufficient to activate IX gene expression. The viral DNA template must first replicate before IX transcription can begin.

MeSH Terms
Adenoviruses, Human/genetics,physiology DNA Replication Gene Expression Regulation Genes, Viral Kinetics RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Templates, Genetic Viral Proteins/genetics Virus Replication
Chemicals
RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Crossland L D
Raskas H J
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.46.3.737-748.1983
Published
1983-06-00
Pages
737-48
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256550
Subset
IM
Grants
NCI NIH HHS · CA-16007 · United States
NCI NIH HHS · CA-16217 · United States
External Links
PubMed source DOI: 10.1128/JVI.46.3.737-748.1983 PMC Full Text View journal details
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