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PMID: 2498646 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An AP1-binding site in the c-fos gene can mediate induction by epidermal growth factor and 12-O-tetradecanoyl phorbol-13-acetate.

Molecular and cellular biology ·Vol. 9 ·No. 3 ·1989-03-00 ·Pages 1327-31

Fisch TM, Prywes R, Roeder RG

Abstract

We have demonstrated that two sequence elements in the c-fos promoter can mediate the response of the gene to epidermal growth factor and the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). The first is the previously described serum response element. The second is a sequence element highly homologous to the consensus binding site for the HeLa cell transcription factor AP1. Although recent reports have shown that fos protein binds to AP1-binding sites through an interaction with AP1 protein and have raised the speculation that fos protein may negatively regulate expression of the c-fos gene via this interaction, we found no role for the AP1 consensus homology in the downregulation of c-fos expression following induction by epidermal growth factor and TPA.

MeSH Terms
Binding Sites Cloning, Molecular DNA-Binding Proteins/metabolism Epidermal Growth Factor/pharmacology Gene Expression Regulation/drug effects HeLa Cells/drug effects,metabolism Humans Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Proto-Oncogenes/drug effects Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors Epidermal Growth Factor Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fisch T M
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Prywes R
Roeder R G
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30 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.3.1327-1331.1989
Published
1989-03-00
Pages
1327-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362726
Subset
IM
Grants
NCI NIH HHS · CA42567 · United States
NIGMS NIH HHS · GM07739 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.3.1327-1331.1989 PMC Full Text View journal details
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