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PMID: 2957694 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Promoter trans-activation of protooncogenes c-fos and c-myc, but not c-Ha-ras, by products of adenovirus early region 1A.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 18 ·1987-09-00 ·Pages 6430-3

Sassone-Corsi P, Borrelli E

Abstract

The E1A (early region 1A) oncogene products of adenovirus type 2 trans-activate the other early viral transcription units, as well as some cellular promoters. Using a short-term cotransfection assay in murine NIH 3T3 fibroblasts, we show that c-fos and c-myc promoter activities are stimulated by the E1A proteins, whereas c-Ha-ras transcription is not affected. The product of E1A 13S mRNA is responsible for the trans-activation, whereas the 12S mRNA product has no effect. Analysis of the c-fos promoter sequences required for the E1A stimulation shows that responsive sequences are located between positions -402 and -240 upstream of the transcription initiation site. This same region also contains the c-fos serum-responsive element. Furthermore, transcription of the endogenous c-fos gene in HeLa cells is increased after E1A transfection.

MeSH Terms
Adenovirus Early Proteins Cell Nucleus/physiology Gene Expression Regulation Oncogene Proteins, Viral/genetics Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogenes RNA, Messenger/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral Proto-Oncogene Proteins RNA, Messenger Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sassone-Corsi P
Borrelli E
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.18.6430
Published
1987-09-00
Pages
6430-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299090
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.18.6430 PMC Full Text View journal details
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