Home LiteratureArticle Details
PMID: 2939459 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The immunoglobulin heavy chain enhancer is stimulated by the adenovirus type 2 E1A products in mouse fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 9 ·1986-05-00 ·Pages 2846-9

Borrelli E, Hen R, Wasylyk C, Wasylyk B, Chambon P

Abstract

In contrast with our previous results (Hen, R., Borrelli, E. & Chambon, P. (1985) Science 230, 1391-1394), which demonstrated that the mouse immunoglobulin heavy chain transcriptional enhancer is repressed in lymphoid cells by the products of the adenovirus type 2 E1A transcription unit, we show here that these products activate the same enhancer in mouse fibroblast L cell lines that contain stably integrated copies of a recombinant in which the enhancer is inserted upstream from the chicken conalbumin promoter. In addition, competition experiments suggest that the activity of the heavy chain enhancer may be repressed by a trans-acting factor in mouse L cells. We speculate that the E1A products may prevent the action of this cellular repressor in these cells.

MeSH Terms
Adenovirus Early Proteins Animals Enhancer Elements, Genetic Fibroblasts/metabolism Genes, Regulator Immunoglobulin Heavy Chains/genetics L Cells/metabolism Mice Oncogene Proteins, Viral/pharmacology Repressor Proteins/physiology Transcription, Genetic
Chemicals
Adenovirus Early Proteins Immunoglobulin Heavy Chains Oncogene Proteins, Viral Repressor Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Borrelli E
Hen R
Wasylyk C
Wasylyk B
Chambon P
References (19)
19 references, click to expand
  1. Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment.
    J Exp Med. 1984 Dec 1;160(6):1937-42 PMID: 6512493
  2. Adenovirus-2 E1A products repress enhancer-induced stimulation of transcription.
    Nature. 1984 Dec 13-19;312(5995):608-12 PMID: 6095113
  3. A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro.
    Nature. 1985 Feb 7-13;313(6002):458-63 PMID: 2982105
  4. Adenovirus E1a proteins repress transcription from the SV40 early promoter.
    Cell. 1985 Mar;40(3):705-16 PMID: 2982503
  5. HeLa cell beta-tubulin gene transcription is stimulated by adenovirus 5 in parallel with viral early genes by an E1a-dependent mechanism.
    Mol Cell Biol. 1984 Dec;4(12):2792-801 PMID: 6527689
  6. Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.
    Mol Cell Biol. 1984 Dec;4(12):2929-31 PMID: 6098829
  7. Epidermal growth factor or serum stimulation of rat fibroblasts induces an elevation in mRNA levels for lactate dehydrogenase and other glycolytic enzymes.
    Nucleic Acids Res. 1985 Feb 11;13(3):711-26 PMID: 3873645
  8. Cell type-specific transcriptional enhancement in vitro requires the presence of trans-acting factors.
    EMBO J. 1985 Nov;4(11):3005-13 PMID: 2998773
  9. Repression of the immunoglobulin heavy chain enhancer by the adenovirus-2 E1A products.
    Science. 1985 Dec 20;230(4732):1391-4 PMID: 2999984
  10. Mouse histocompatibility genes: structure and organisation of a Kd gene.
    EMBO J. 1983;2(2):245-54 PMID: 11894934
  11. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
    Cell. 1979 Jul;17(3):683-9 PMID: 476833
  12. Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5684-8 PMID: 293672
  13. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  14. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  15. Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product.
    Cell. 1982 Jul;29(3):913-9 PMID: 7151177
  16. Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7132-6 PMID: 6961401
  17. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  18. Identification and mapping of polypeptides encoded by the P3HR-1 strain of Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4183-7 PMID: 6330747
  19. B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo.
    Science. 1985 Jan 11;227(4683):134-40 PMID: 3917574
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.9.2846
Published
1986-05-00
Pages
2846-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323403
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.9.2846 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2