Home LiteratureArticle Details

PMID: 6098829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.

Molecular and cellular biology ·Vol. 4 ·No. 12 ·1984-12-00 ·Pages 2929-31

Blochlinger K, Diggelmann H

Abstract

The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.

MeSH Terms

Cell Line DNA Restriction Enzymes/metabolism Drug Resistance Escherichia coli/genetics Hygromycin B/pharmacology Kanamycin Kinase Moloney murine sarcoma virus/genetics Phosphotransferases/genetics Repetitive Sequences, Nucleic Acid Sarcoma Viruses, Murine/genetics Transfection

Chemicals

Hygromycin B Phosphotransferases Kanamycin Kinase DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Blochlinger K

Diggelmann H

References (13)

13 references, click to expand

Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.
Gene. 1983 Nov;25(2-3):179-88 PMID: 6319235
Initiation of translation at internal AUG codons in mammalian cells.
Nature. 1984 May 3-9;309(5963):82-5 PMID: 6717585
Molecularly cloned c-mos(rat) is biologically active.
EMBO J. 1982;1(11):1313-7 PMID: 6327263
Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin.
Nucleic Acids Res. 1984 May 11;12(9):3873-93 PMID: 6328442
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition.
Eur J Biochem. 1978 Jun 1;87(1):21-7 PMID: 208837
Phenotypic suppression and misreading Saccharomyces cerevisiae.
Nature. 1979 Jan 11;277(5692):146-8 PMID: 366438
Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.
Biochim Biophys Acta. 1978 Dec 21;521(2):459-69 PMID: 367435
Number and location of mouse mammary tumor virus proviral DNA in mouse DNA of normal tissue and of mammary tumors.
J Virol. 1980 Mar;33(3):1013-25 PMID: 6245257
Nucleotide sequence of the genome of a murine sarcoma virus.
Cell. 1981 Nov;27(1 Pt 2):97-108 PMID: 6173134
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
Molecular basis of the permeabilization of mammalian cells by ionophores.
Eur J Biochem. 1982 Oct;127(3):567-9 PMID: 7173196
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
Methods Enzymol. 1983;100:255-66 PMID: 6194404

Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.4.12.2929-2931.1984

Published

1984-12-00

Pages

2929-31

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC369308

Subset

External Links

PubMed source DOI: 10.1128/mcb.4.12.2929-2931.1984 PMC Full Text View journal details

Analysis Services

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.

Contact

WeChat Official Account