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PMID: 8440235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A nucleosome-dependent static loop potentiates estrogen-regulated transcription from the Xenopus vitellogenin B1 promoter in vitro.

The EMBO journal ·Vol. 12 ·No. 2 ·1993-02-00 ·Pages 423-33

Schild C, Claret FX, Wahli W, Wolffe AP

Abstract

We describe the transcriptional potentiation in estrogen responsive transcription extracts of the Xenopus vitellogenin B1 gene promoter through the formation of a positioned nucleosome. Nuclease digestion and hydroxyl radical cleavage indicate that strong, DNA sequence-directed positioning of a nucleosome occurs between -300 and -140 relative to the start site of transcription. Deletion of this DNA sequence abolishes the potentiation of transcription due to nucleosome assembly. The wrapping of DNA around the histone core of the nucleosome positioned between -300 and -140 creates a static loop in which distal estrogen receptor binding sites are brought close to proximal promoter elements. This might facilitate interactions between the trans-acting factors themselves and/or RNA polymerase. Such a nucleosome provides an example of how chromatin structure might have a positive effect on the transcription process.

MeSH Terms
Animals Base Sequence Chromatin DNA, Single-Stranded Estrogens/physiology Female Gene Expression Regulation HeLa Cells Humans Molecular Sequence Data Nucleic Acid Conformation Nucleosomes/metabolism Promoter Regions, Genetic Receptors, Estrogen/metabolism Transcription Factors/metabolism Transcription, Genetic Vitellogenins/genetics Xenopus
Chemicals
Chromatin DNA, Single-Stranded Estrogens Nucleosomes Receptors, Estrogen Transcription Factors Vitellogenins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schild C
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892.
Claret F X
Wahli W
Wolffe A P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-02-00
Pages
423-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413225
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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