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PMID: 2236004 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Estrogen receptor level determines sex-specific in vitro transcription from the Xenopus vitellogenin promoter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 20 ·1990-10-00 ·Pages 7878-82

Corthésy B, Claret FX, Wahli W

Abstract

Female-specific expression of the Xenopus laevis vitellogenin gene was reconstituted in vitro by addition of recombinant vaccinia-virus-produced estrogen receptor to nuclear extracts from male livers, in which this gene is silent. Transcription enhancement was at least 30 times and was selectively restricted to vitellogenin templates containing the estrogen-responsive unit. Thus, in male hepatocytes, estrogen receptor is the limiting regulatory factor that in the female liver controls efficient and accurate sex-specific expression of the vitellogenin gene. Furthermore, the Xenopus liver factor B, which is essential in addition to the estrogen receptor for the activation of this gene, was successfully replaced in the Xenopus extract by purified human nuclear factor I, identifying factor B of Xenopus as a functional homolog of this well-characterized human transcription factor.

MeSH Terms
Animals Base Sequence CCAAT-Enhancer-Binding Proteins Cell Nucleus/metabolism Chromosome Deletion DNA-Binding Proteins/metabolism Female Humans Male Molecular Sequence Data Mutagenesis, Site-Directed NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Promoter Regions, Genetic Receptors, Estrogen/physiology Restriction Mapping Sex Factors Templates, Genetic Transcription Factors Transcription, Genetic Vitellogenins/genetics Xenopus laevis Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Receptors, Estrogen Transcription Factors Vitellogenins Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Corthésy B
Insitut de Biologie Animale, Université de Lausanne, Switzerland.
Claret F X
Wahli W
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.20.7878
Published
1990-10-00
Pages
7878-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54854
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.20.7878 PMC Full Text View journal details
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