A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 689-96

Simon RH, Felsenfeld G

Abstract

A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA.

MeSH Terms

Animals Chickens Chromatin/analysis Chromatography/methods Ducks Erythrocytes/analysis Histones/blood,isolation & purification Hydroxyapatites

Chemicals

Chromatin Histones Hydroxyapatites

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Simon R H

Felsenfeld G

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.2.689

Published

1979-02-00

Pages

689-96

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327721

Subset

External Links

PubMed source DOI: 10.1093/nar/6.2.689 PMC Full Text View journal details

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A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.

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