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PMID: 3402432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro.

The EMBO journal ·Vol. 7 ·No. 4 ·1988-04-00 ·Pages 1071-9

Wolffe AP

Abstract

An extract of whole oocytes (oocyte S150) differentially transcribes Xenopus oocyte and somatic 5S RNA genes. In the oocyte S150, transcription complexes with different stabilities are assembled onto oocyte and somatic 5S DNA. The stability of the transcription complex is dependent on activities present in a fraction containing transcription factor TFIIIC. This fraction stabilizes the binding of the positive transcription factor TFIIIA to a somatic 5S RNA gene much more efficiently than it does to an oocyte gene. The oocyte S150 transcription extract is deficient in TFIIIC such that supplementation with a fraction enriched in this transcription factor selectively stimulates oocyte 5S DNA transcription. Previously it has been shown that an egg extract deficient in TFIIIA selectively transcribes somatic 5S RNA genes. Thus under conditions where there is differential stability of transcription complexes, limitation of either TFIIIA or TFIIIC may exaggerate the differential expression of two genes.

MeSH Terms
Animals Female Gene Expression Regulation Genes Oocytes/metabolism Plasmids Protein Binding RNA Polymerase III/metabolism RNA, Ribosomal/genetics RNA, Ribosomal, 5S/genetics Transcription Factors/metabolism Transcription Factors, TFIII Transcription, Genetic Xenopus laevis
Chemicals
RNA, Ribosomal RNA, Ribosomal, 5S Transcription Factors Transcription Factors, TFIII transcription factor TFIIIC RNA Polymerase III
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wolffe A P
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-04-00
Pages
1071-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454438
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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