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PMID: 3466137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.

Nucleic acids research ·Vol. 14 ·No. 22 ·1986-11-25 ·Pages 8755-70

Seiler-Tuyns A, Walker P, Martinez E, Mérillat AM, Givel F, Wahli W

Abstract

The expression of a hybrid gene formed by the promoter region of the Xenopus laevis vitellogenin gene B1 and the CAT coding region is regulated by estrogen when the gene is transfected into hormone-responsive MCF-7 cells. Furthermore, the 5' flanking region of the gene B1 alone can confer inducibility to heterologous promoters, although to a varying extent depending on the promoter used. Deletion mapping of he vitellogenin hormone-responsive sequences revealed that a 13 bp element 5'-AGTCACTGTGACC-3' at position -334 is essential for estrogen inducibility. We have shown previously that this 13 bp element is present upstream of several liver-specific estrogen-inducible genes.

MeSH Terms
Acetyltransferases/genetics Animals Breast Neoplasms/genetics,metabolism Cell Line Chloramphenicol O-Acetyltransferase Chromosome Deletion Estradiol/pharmacology Female Genes/drug effects Globins/genetics Humans Mutation Plasmids Promoter Regions, Genetic Transcription, Genetic/drug effects Vitellogenins/genetics Xenopus
Chemicals
Vitellogenins Estradiol Globins Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Seiler-Tuyns A
Walker P
Martinez E
Mérillat A M
Givel F
Wahli W
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.22.8755
Published
1986-11-25
Pages
8755-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311909
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.22.8755 PMC Full Text View journal details
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