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PMID: 6294657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vitellogenin genes A1 and B1 are linked in the Xenopus laevis genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 22 ·1982-11-00 ·Pages 6832-6

Wahli W, Germond JE, ten Heggeler B, May FE

Abstract

Genomic clones containing the Xenopus laevis vitellogenin gene B1 have been isolated from DNA libraries and characterized by heteroduplex mapping in the electron microscope, restriction endonuclease analysis, and in vitro transcription in a HeLa whole-cell extract. Sequences from the 3'-flanking region of the previously isolated A1 vitellogenin gene were found in the 5'-flanking region of this B1 gene. Thus, the two genes are linked, with 15.5 kilobase pairs of DNA between them. Their length is about 22 kilobase pairs (A1 gene) and 16.5 kilobase pairs (B1 gene) and they have the following arrangement: 5'-A1 gene-spacer-B1 gene-3'. The analysis of heteroduplexes formed between the two genes revealed several regions of homology. Both genes are in the same orientation and, therefore, are transcribed from the same DNA strand. The possible events by which the vitellogenin gene family arose in Xenopus laevis are discussed.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Female Genes Genetic Linkage Lipoproteins/genetics Microscopy, Electron Transcription, Genetic Vitellogenins/genetics Xenopus
Chemicals
DNA, Recombinant Lipoproteins Vitellogenins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wahli W
Germond J E
ten Heggeler B
May F E
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.22.6832
Published
1982-11-00
Pages
6832-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347227
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.22.6832 PMC Full Text View journal details
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