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PMID: 2038313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synergistic transcriptional activation by CTF/NF-I and the estrogen receptor involves stabilized interactions with a limiting target factor.

Molecular and cellular biology ·Vol. 11 ·No. 6 ·1991-06-00 ·Pages 2937-45

Martinez E, Dusserre Y, Wahli W, Mermod N

Abstract

Transcription initiation at eukaryotic protein-coding gene promoters is regulated by a complex interplay of site-specific DNA-binding proteins acting synergistically or antagonistically. Here, we have analyzed the mechanisms of synergistic transcriptional activation between members of the CCAAT-binding transcription factor/nuclear factor I (CTF/NF-I) family and the estrogen receptor. By using cotransfection experiments with HeLa cells, we show that the proline-rich transcriptional activation domain of CTF-1, when fused to the GAL4 DNA-binding domain, synergizes with each of the two estrogen receptor-activating regions. Cooperative DNA binding between the GAL4-CTF-1 fusion and the estrogen receptor does not occur in vitro, and in vivo competition experiments demonstrate that both activators can be specifically inhibited by the overexpression of a proline-rich competitor, indicating that a common limiting factor is mediating their transcriptional activation functions. Furthermore, the two activators functioning synergistically are much more resistant to competition than either factor alone, suggesting that synergism between CTF-1 and the estrogen receptor is the result of a stronger tethering of the limiting target factor(s) to the two promoter-bound activators.

MeSH Terms
CCAAT-Enhancer-Binding Proteins Chloramphenicol O-Acetyltransferase/genetics,metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation HeLa Cells/physiology Humans Kinetics Models, Genetic NFI Transcription Factors Nuclear Proteins Plasmids Proline Promoter Regions, Genetic Receptors, Estrogen/metabolism Restriction Mapping Transcription Factors Transcription, Genetic Transfection Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors NFIC protein, human Nuclear Proteins Receptors, Estrogen Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human Proline Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Martinez E
Institut de Biologie Animale, Bâtiment de Biologie, Université de Lausanne, Switzerland.
Dusserre Y
Wahli W
Mermod N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.6.2937-2945.1991
Published
1991-06-00
Pages
2937-45
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360120
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.6.2937-2945.1991 PMC Full Text View journal details
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