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PMID: 2690074 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Initiation of transcription on nucleosomal templates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 9817-21

Wolffe AP, Drew HR

Abstract

We describe a simple method that uses curved DNAs to move nucleosomes relative to a sequence of interest. With this method, small changes in the association of a T7 RNA polymerase promoter with a nucleosome are shown to lead to significant changes in transcription efficiency.

MeSH Terms
Animals Base Sequence Chickens Cloning, Molecular DNA/chemical synthesis,genetics Deoxyribonuclease I Erythrocytes/metabolism Genetic Techniques Histones/metabolism Molecular Sequence Data Nucleic Acid Conformation Nucleosomes/metabolism Nucleotide Mapping Restriction Mapping Templates, Genetic Transcription, Genetic
Chemicals
Histones Nucleosomes DNA Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wolffe A P
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Drew H R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.24.9817
Published
1989-12-00
Pages
9817-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298593
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.24.9817 PMC Full Text View journal details
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