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PMID: 1650485 Published · ppublish English Journal Article

Histone contributions to the structure of DNA in the nucleosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 15 ·1991-08-01 ·Pages 6829-33

Hayes JJ, Clark DJ, Wolffe AP

Abstract

We describe the application of the hydroxyl radical footprinting technique to examine the contribution of the core histone tails and of histones H3 and H4 to the structure of DNA in the nucleosome. We first establish that, as was previously determined for a nucleosome containing a unique sequence of DNA, mixed-sequence nucleosomes contain two distinct regions of DNA structure. The central three turns of DNA in the nucleosome have a helical periodicity of approximately 10.7 base pairs per turn, while flanking regions have a periodicity of approximately 10.0 base pairs per turn. Removal of the histone tails does not change the hydroxyl radical cleavage pattern in either mixed- or unique-sequence nucleosome samples. A tetramer of histones H3 and H4, (H3/H4)2, organizes the central 120 base pairs of DNA identically to that found in the nucleosome. Moreover, "tailless" octamers and the (H3/H4)2 tetramer recognize the same nucleosome positioning signals as the intact octamer.

MeSH Terms
Animals DNA/isolation & purification,metabolism DNA, Ribosomal/metabolism,ultrastructure Deoxyribonuclease I Free Radicals Histones/isolation & purification,metabolism,ultrastructure Hydroxides Hydroxyl Radical Macromolecular Substances Nucleosomes/metabolism,ultrastructure RNA, Ribosomal, 5S/genetics Restriction Mapping Xenopus
Chemicals
DNA, Ribosomal Free Radicals Histones Hydroxides Macromolecular Substances Nucleosomes RNA, Ribosomal, 5S Hydroxyl Radical DNA Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hayes J J
Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Clark D J
Wolffe A P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.15.6829
Published
1991-08-01
Pages
6829-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52182
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.15.6829 PMC Full Text View journal details
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