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PMID: 2170977 Published · ppublish English Journal Article

The structure of DNA in a nucleosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 19 ·1990-10-00 ·Pages 7405-9

Hayes JJ, Tullius TD, Wolffe AP

Abstract

We describe the application of the hydroxyl radical footprinting technique to examine the histone-DNA interactions of a nucleosome that includes part of the 5S ribosomal RNA gene of Xenopus borealis. We establish that two distinct regions of DNA with different helical periodicities exist within the nucleosome and demonstrate a change in the helical periodicity of this DNA upon nucleosome formation. In particular, we find that on average the helical periodicity of DNA in this nucleosome is 10.18 +/- 0.05 base pairs per turn. The same DNA, when bound to a calcium phosphate surface, has a periodicity of 10.49 +/- 0.05 base pairs per turn, similar to that of random sequence DNA. Modulations in minor groove width within the naked DNA detected by the hydroxyl radical are maintained and exaggerated in nucleosomal DNA. These features correlate with regions in the DNA previously suggested to be important for nucleosome positioning.

MeSH Terms
Animals DNA/analysis,metabolism DNA, Ribosomal/analysis,genetics Fourier Analysis Free Radicals Hydroxides Hydroxyl Radical Nucleic Acid Conformation Nucleosomes/chemistry,ultrastructure Nucleotide Mapping RNA, Ribosomal, 5S/genetics Restriction Mapping Xenopus
Chemicals
DNA, Ribosomal Free Radicals Hydroxides Nucleosomes RNA, Ribosomal, 5S Hydroxyl Radical DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hayes J J
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Tullius T D
Wolffe A P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.19.7405
Published
1990-10-00
Pages
7405-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54755
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.19.7405 PMC Full Text View journal details
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