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PMID: 1406639 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 12 ·No. 10 ·1992-10-00 ·Pages 4503-14

Jiang YW, Stillman DJ

Abstract

We have cloned and sequenced the SIN4 gene and determined that SIN4 is identical to TSF3, identified as a negative regulator of GAL1 gene transcription (S. Chen, R.W. West, Jr., S.L. Johnson, H. Gans, and J. Ma, submitted for publication). Yeast strains bearing a sin4 delta null mutation have been constructed and are temperature sensitive for growth and display defects in both negative and positive regulation of transcription. Transcription of the CTS1 gene is reduced in sin4 delta mutants, suggesting that Sin4 functions as a positive transcriptional regulator. Additionally, a Sin4-LexA fusion protein activates transcription from test promoters containing LexA binding sites. The sin4 delta mutant also shows phenotypes common to histone and spt mutants, including suppression of delta insertion mutations in the HIS4 and LYS2 promoters, expression of promoters lacking upstream activation sequence elements, and decreased superhelical density of circular DNA molecules. These results suggest that the sin4 delta mutation may alter the structure of chromatin, and these changes in chromatin structure may affect transcriptional regulation.

MeSH Terms
Amino Acid Sequence Base Sequence Chromatin/ultrastructure Cloning, Molecular DNA, Fungal Fungal Proteins/genetics,metabolism Genes, Fungal Mediator Complex Molecular Sequence Data Mutagenesis Phenotype Promoter Regions, Genetic Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Trans-Activators/genetics,metabolism
Chemicals
Chromatin DNA, Fungal Fungal Proteins Mediator Complex SIN4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jiang Y W
Department of Cellular, Viral, and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132.
Stillman D J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.10.4503-4514.1992
Published
1992-10-00
Pages
4503-14
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360376
Subset
IM
Databases
GENBANK
M93050
External Links
PubMed source DOI: 10.1128/mcb.12.10.4503-4514.1992 PMC Full Text View journal details
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