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PMID: 3143101 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain.

Nucleic acids research ·Vol. 16 ·No. 21 ·1988-11-11 ·Pages 10153-69

O'Hara PJ, Horowitz H, Eichinger G, Young ET

Abstract

The ADR6 gene of Saccharomyces cerevisiae has an open reading frame which could encode a polypeptide of 1314 amino acids. The predicted mRNA encodes a protein with homopolymeric stretches of asparagine and threonine, particularly near its amino terminus and contains additional sequences consisting of polyglutamine repeats. The predicted protein also contains a potential metal binding (Cys)4-type finger near its carboxy-terminus. An ADR6/beta-galactosidase fusion protein was predominantly nuclear in location, consistent with its role as an activator of ADH2 transcription.

MeSH Terms
Alcohol Dehydrogenase/genetics Amino Acid Sequence Base Sequence DNA-Binding Proteins/genetics Genes Genes, Fungal Genes, Regulator Metalloproteins/genetics Molecular Sequence Data RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic Zinc/metabolism
Chemicals
DNA-Binding Proteins Metalloproteins RNA, Messenger Alcohol Dehydrogenase Zinc
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
O'Hara P J
ZymoGenetics, Inc., Seattle, WA 98105.
Horowitz H
Eichinger G
Young E T
References (83)
83 references, click to expand
  1. opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster.
    Cell. 1985 Jan;40(1):55-62 PMID: 2981631
  2. Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA.
    EMBO J. 1985 Feb;4(2):465-73 PMID: 4040462
  3. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
    Science. 1987 Nov 6;238(4828):797-9 PMID: 3672127
  4. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  5. Coding potential and regulatory signals of the polyoma virus genome.
    Nature. 1980 Jan 31;283(5746):445-53 PMID: 6243401
  6. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  7. Isolation and amino acid sequences of proline-rich peptides of human whole saliva.
    J Biochem. 1979 Jul;86(1):79-86 PMID: 479131
  8. Targeting of E. coli beta-galactosidase to the nucleus in yeast.
    Cell. 1984 Apr;36(4):1057-65 PMID: 6323016
  9. The Notch locus of Drosophila melanogaster.
    Cell. 1983 Sep;34(2):421-33 PMID: 6193889
  10. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  11. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
    Nature. 1984 Jul 5-11;310(5972):25-31 PMID: 6330566
  12. LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.
    Mol Cell Biol. 1987 Aug;7(8):2708-17 PMID: 2823102
  13. Nucleotide sequence of the ARGRII regulatory gene and amino acid sequence homologies between ARGRII PPRI and GAL4 regulatory proteins.
    Eur J Biochem. 1986 May 15;157(1):77-81 PMID: 3709534
  14. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
    Methods Enzymol. 1983;100:293-308 PMID: 6312261
  15. Profile analysis: detection of distantly related proteins.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4355-8 PMID: 3474607
  16. Structural organization and sequence of the homeotic gene Antennapedia of Drosophila melanogaster.
    EMBO J. 1986 Apr;5(4):733-9 PMID: 10408949
  17. Human polyomavirus JC virus genome.
    J Virol. 1984 Aug;51(2):458-69 PMID: 6086957
  18. DNA sequences, gene regulation and modular protein evolution in the Drosophila 68C glue gene cluster.
    J Mol Biol. 1983 Aug 25;168(4):765-89 PMID: 6411930
  19. Using PC/GENE for protein and nucleic acid analysis.
    Biotechniques. 1988 Jun;6(6):566-72 PMID: 3273189
  20. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  21. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
    J Cell Biol. 1984 Mar;98(3):922-33 PMID: 6365930
  22. The yeast his3 promoter contains at least two distinct elements.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7385-9 PMID: 6760196
  23. Metal-binding, nucleic acid-binding finger sequences in the CDC16 gene of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1987 Oct 26;15(20):8439-50 PMID: 2823230
  24. The protein identification resource (PIR).
    Nucleic Acids Res. 1986 Jan 10;14(1):11-5 PMID: 3945547
  25. Identification and consequences of a guanosine-15 to adenosine-15 change in the yeast mitochondrial tRNASerUCX gene.
    Biochemistry. 1983 Mar 29;22(7):1709-14 PMID: 6342666
  26. The genome of human papovavirus BKV.
    Cell. 1979 Dec;18(4):963-77 PMID: 229976
  27. A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.
    EMBO J. 1985 May;4(5):1279-86 PMID: 2988942
  28. The engrailed locus of Drosophila: structural analysis of an embryonic transcript.
    Cell. 1985 Jan;40(1):37-43 PMID: 3917855
  29. Transcriptional analysis of the Saccharomyces cerevisiae mitochondrial var1 gene: anomalous hybridization of RNA from AT-rich regions.
    Mol Cell Biol. 1983 Sep;3(9):1615-24 PMID: 6355829
  30. Developmental and molecular analysis of Deformed; a homeotic gene controlling Drosophila head development.
    EMBO J. 1987 Mar;6(3):767-77 PMID: 16453752
  31. Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes.
    Nucleic Acids Res. 1986 Jul 11;14(13):5125-43 PMID: 3526280
  32. Amino acid sequences of mouse and human epidermal type II keratins of Mr 67,000 provide a systematic basis for the structural and functional diversity of the end domains of keratin intermediate filament subunits.
    J Biol Chem. 1985 Jun 10;260(11):7142-9 PMID: 2581964
  33. Zinc fingers: gilt by association.
    Cell. 1988 Jan 15;52(1):1-3 PMID: 3125980
  34. Structure and sequence of the Drosophila zeste gene.
    EMBO J. 1987 Mar;6(3):791-9 PMID: 3582372
  35. The cloning and mapping of ADR6, a gene required for sporulation and for expression of the alcohol dehydrogenase II isozyme from Saccharomyces cerevisiae.
    Genetics. 1987 Aug;116(4):531-40 PMID: 3040523
  36. Isolation and characterization of the positive regulatory gene ADR1 from Saccharomyces cerevisiae.
    Mol Cell Biol. 1983 Mar;3(3):360-70 PMID: 6341814
  37. Structural analysis of murine genes containing homoeo box sequences and their expression in embryonal carcinoma cells.
    Nature. 1985 Apr 25-May 1;314(6013):713-8 PMID: 2986010
  38. The yeast regulatory gene PHO2 encodes a homeo box.
    Cell. 1988 May 6;53(3):339-40 PMID: 2896548
  39. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  40. Potential metal-binding domains in nucleic acid binding proteins.
    Science. 1986 Apr 25;232(4749):485-7 PMID: 2421409
  41. Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs.
    Nucleic Acids Res. 1987 Apr 24;15(8):3581-93 PMID: 3554144
  42. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes.
    Nature. 1984 Mar 29-Apr 4;308(5958):428-33 PMID: 6323992
  43. Primary structure of the Saccharomyces cerevisiae GAL4 gene.
    Mol Cell Biol. 1984 Feb;4(2):260-7 PMID: 6366516
  44. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  45. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  46. The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts.
    Mol Cell Biol. 1988 Feb;8(2):655-63 PMID: 2832732
  47. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  48. The Drosophila single-minded gene encodes a nuclear protein with sequence similarity to the per gene product.
    Cell. 1988 Jan 15;52(1):143-51 PMID: 3345560
  49. Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene.
    EMBO J. 1988 Feb;7(2):485-94 PMID: 3284746
  50. Nitrogen fixation specific regulatory genes of Klebsiella pneumoniae and Rhizobium meliloti share homology with the general nitrogen regulatory gene ntrC of K. pneumoniae.
    Nucleic Acids Res. 1985 Jun 25;13(12):4539-55 PMID: 2989799
  51. (TAA)n within sequences flanking several intrachromosomal variant surface glycoprotein genes in Trypanosoma brucei.
    Nucleic Acids Res. 1985 May 10;13(9):3161-77 PMID: 2987874
  52. Deletion analysis identifies a region, upstream of the ADH2 gene of Saccharomyces cerevisiae, which is required for ADR1-mediated derepression.
    Mol Cell Biol. 1985 Jul;5(7):1743-9 PMID: 3160930
  53. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  54. The 5'-limit of transposition and upstream barren region of a trypanosome VSG gene: tandem 76 base-pair repeats flanking (TAA)90.
    Nucleic Acids Res. 1984 Mar 26;12(6):2759-74 PMID: 6324125
  55. Location and structure of the var1 gene on yeast mitochondrial DNA: nucleotide sequence of the 40.0 allele.
    Cell. 1982 Sep;30(2):617-26 PMID: 6754087
  56. The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual aminoacid composition.
    Nucleic Acids Res. 1987 Jan 12;15(1):233-46 PMID: 3029672
  57. Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone.
    EMBO J. 1984 Jun;3(6):1409-15 PMID: 6204862
  58. Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: structural and functional relationships to GAL4 of Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Mar;7(3):1111-21 PMID: 3550430
  59. Identification of functional regions in the yeast transcriptional activator ADR1.
    Mol Cell Biol. 1988 May;8(5):2125-31 PMID: 3290650
  60. Nucleotide sequence of a cDNA clone encoding mouse protamine 1.
    Biochemistry. 1985 Jan 29;24(3):719-22 PMID: 2986684
  61. Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats.
    Cell. 1985 Dec;43(3 Pt 2):567-81 PMID: 3935325
  62. Primary structure and developmental expression pattern of Hox 3.1, a member of the murine Hox 3 homeobox gene cluster.
    EMBO J. 1988 May;7(5):1329-36 PMID: 2900757
  63. Isolation, structure, and expression of even-skipped: a second pair-rule gene of Drosophila containing a homeo box.
    Cell. 1986 Dec 5;47(5):721-34 PMID: 2877745
  64. Three novel genes of human T-lymphotropic virus type III: immune reactivity of their products with sera from acquired immune deficiency syndrome patients.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2209-13 PMID: 3008154
  65. Sequence and nuclear localization of the Saccharomyces cerevisiae HAP2 protein, a transcriptional activator.
    Mol Cell Biol. 1987 Feb;7(2):578-85 PMID: 3547076
  66. Cell-free synthesis of rat parotid preamylase.
    J Biol Chem. 1979 Jan 25;254(2):525-9 PMID: 310817
  67. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  68. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  69. Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.
    Mol Cell Biol. 1987 Mar;7(3):1256-66 PMID: 2951591
  70. Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage.
    J Mol Biol. 1984 Dec 5;180(2):239-50 PMID: 6096561
  71. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  72. var1 Gene on the mitochondrial genome of Torulopsis glabrata.
    J Mol Biol. 1985 Aug 20;184(4):565-76 PMID: 3900417
  73. The GenBank genetic sequence databank.
    Nucleic Acids Res. 1986 Jan 10;14(1):1-4 PMID: 3945546
  74. A Plasmodium falciparum antigen containing clusters of asparagine residues.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2677-81 PMID: 3517875
  75. Organization and structure of the mouse interleukin-2 gene.
    Nucleic Acids Res. 1984 Dec 21;12(24):9323-31 PMID: 6240025
  76. Molecular cloning and characterization of winter flounder antifreeze cDNA.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2825-9 PMID: 6265915
  77. Amino acid sequence of phosvitin derived from the nucleotide sequence of part of the chicken vitellogenin gene.
    Biochemistry. 1984 Sep 11;23(19):4275-9 PMID: 6091745
  78. An asparagine-rich protein from blood stages of Plasmodium falciparum shares determinants with sporozoites.
    Nucleic Acids Res. 1986 Apr 11;14(7):3089-102 PMID: 2421257
  79. The identification and characterization of ADR6, a gene required for sporulation and for expression of the alcohol dehydrogenase II isozyme from Saccharomyces cerevisiae.
    Genetics. 1987 Aug;116(4):523-30 PMID: 3305157
  80. Expression of the caudal gene in the germ line of Drosophila: formation of an RNA and protein gradient during early embryogenesis.
    Cell. 1987 Feb 13;48(3):465-78 PMID: 2433048
  81. The EMBL data library.
    Nucleic Acids Res. 1986 Jan 10;14(1):5-9 PMID: 3945550
  82. pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1819-23 PMID: 3031652
  83. Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA.
    Nature. 1986 Mar 20-26;320(6059):283-7 PMID: 3515197
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.21.10153
Published
1988-11-11
Pages
10153-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338843
Subset
IM
Grants
NIGMS NIH HHS · GM26079 · United States
Databases
GENBANK
X12493
External Links
PubMed source DOI: 10.1093/nar/16.21.10153 PMC Full Text View journal details
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