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PMID: 2951591 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.

Molecular and cellular biology ·Vol. 7 ·No. 3 ·1987-03-00 ·Pages 1256-66

Baum JA, Geever R, Giles NH

Abstract

The qa-1F regulatory gene of Neurospora crassa encodes an activator protein required for quinic acid induction of transcription in the qa gene cluster. This activator protein was expressed in insect cell culture with a baculovirus expression vector. The activator binds to 13 sites in the gene cluster that are characterized by a conserved 16-base-pair sequence of partial dyad symmetry. One site is located between the divergently transcribed qa-1F and qa-1S regulatory genes, corroborating prior evidence that qa-1F is autoregulated and controls expression of the qa-1S repressor. Multiple upstream sites located at variable positions 5' to the qa structural genes appear to allow for greater transcriptional control by qa-1F. Full-length and truncated activator peptides were synthesized in vitro, and the DNA-binding domain was localized to the first 183 amino acids. A 28-amino acid sequence within this region shows striking homology to N-terminal sequences from other lower-eucaryotic activator proteins. A qa-1F(Ts) mutation is located within this putative DNA-binding domain.

MeSH Terms
Animals Binding Sites Cells, Cultured DNA, Fungal/metabolism Fungal Proteins/genetics,metabolism Gene Expression Regulation Genes, Fungal Genes, Regulator Genetic Vectors Insect Viruses/genetics Insecta Multigene Family Mutation Neurospora/genetics Neurospora crassa/genetics,metabolism
Chemicals
DNA, Fungal Fungal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baum J A
Geever R
Giles N H
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39 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.3.1256-1266.1987
Published
1987-03-00
Pages
1256-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365200
Subset
IM
Grants
NIGMS NIH HHS · GM28777 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.3.1256-1266.1987 PMC Full Text View journal details
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