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PMID: 2944110 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNase I hypersensitive sites within the inducible qa gene cluster of Neurospora crassa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6533-7

Baum JA, Giles NH

Abstract

DNase I hypersensitive regions were mapped within the 17.3-kilobase qa (quinic acid) gene cluster of Neurospora crassa. The 5'-flanking regions of the five qa structural genes and the two qa regulatory genes each contain DNase I hypersensitive sites under noninducing conditions and generally exhibit increases in DNase I cleavage upon induction of transcription with quinic acid. The two large intergenic regions of the qa gene cluster appear to be similarly organized with respect to the positions of constitutive and inducible DNase I hypersensitive sites. Inducible hypersensitive sites on the 5' side of one qa gene, qa-x, appear to be differentially regulated. Employing these and previously published data, we have identified a conserved sequence element that may mediate the activator function of the qa-1F regulatory gene. Variants of the 16-base-pair consensus sequence are consistently found within DNase I-protected regions adjacent to inducible DNase I hypersensitive sites within the gene cluster.

MeSH Terms
Chromatin/ultrastructure Chromosome Mapping DNA, Fungal/genetics Deoxyribonuclease I Gene Expression Regulation Neurospora/genetics Neurospora crassa/genetics Quinic Acid/physiology Transcription, Genetic
Chemicals
Chromatin DNA, Fungal Quinic Acid Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baum J A
Giles N H
References (16)
16 references, click to expand
  1. Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli.
    J Mol Appl Genet. 1983;2(1):83-99 PMID: 6221060
  2. A dominant role for DNA secondary structure in forming hypersensitive structures in chromatin.
    Cell. 1983 Apr;32(4):1191-203 PMID: 6301683
  3. Cis-acting and trans-acting regulatory mutations define two types of promoters controlled by the qa-1F gene of Neurospora.
    Cell. 1984 Feb;36(2):493-502 PMID: 6198093
  4. Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9 PMID: 6320177
  5. Molecular analysis of the Neurospora qa-1 regulatory region indicates that two interacting genes control qa gene expression.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1174-8 PMID: 6322189
  6. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  7. Genetic control of chromatin structure 5' to the qa-x and qa-2 genes of Neurospora.
    J Mol Biol. 1985 Mar 5;182(1):79-89 PMID: 3999144
  8. Gene organization and regulation in the qa (quinic acid) gene cluster of Neurospora crassa.
    Microbiol Rev. 1985 Sep;49(3):338-58 PMID: 2931582
  9. Assembly and propagation of repressed and depressed chromosomal states.
    Cell. 1985 Oct;42(3):705-11 PMID: 2996773
  10. The relationship of regulatory proteins and DNase I hypersensitive sites in the yeast GAL1-10 genes.
    Nucleic Acids Res. 1985 Dec 9;13(23):8409-23 PMID: 3909104
  11. Rearrangement mutations on the 5' side of the qa-2 gene of Neurospora implicate two regions of qa-1F activator-protein interaction.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3944-8 PMID: 2940595
  12. Autogenous regulation of the positive regulatory qa-1F gene in Neurospora crassa.
    Mol Cell Biol. 1985 Dec;5(12):3593-9 PMID: 2943985
  13. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  14. Genetic organization and transcriptional regulation in the qa gene cluster of Neurospora crassa.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5783-7 PMID: 6458044
  15. Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe.
    Gene. 1982 Dec;20(3):387-96 PMID: 6299898
  16. Point mutations and DNA rearrangements 5' to the inducible qa-2 gene of Neurospora allow activator protein-independent transcription.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7298-302 PMID: 6316356
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6533
Published
1986-09-00
Pages
6533-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386538
Subset
IM
Grants
NIGMS NIH HHS · GM28777 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.17.6533 PMC Full Text View journal details
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