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PMID: 2900757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Primary structure and developmental expression pattern of Hox 3.1, a member of the murine Hox 3 homeobox gene cluster.

The EMBO journal ·Vol. 7 ·No. 5 ·1988-05-00 ·Pages 1329-36

Breier G, Dressler GR, Gruss P

Abstract

The murine Hox 3.1 gene maps to a cluster of homeobox-containing genes on chromosome 15. We report the primary structure of the Hox 3.1 protein, as deduced from cDNA sequences, and the expression of Hox 3.1 mRNA during embryogenesis. In addition, a second member of the gene cluster, Hox 3.2, is characterized. The predicted Hox 3.1 protein consists of 242 amino acid residues and has a calculated mol. wt of 28 kd. Besides the homeodomain, it shares with other murine homeodomain proteins a conserved hexapeptide, a region rich in glutamic acid residues at the carboxy terminus and homology at the amino terminus. During embryogenesis, Hox 3.1 transcripts are detected first in the posterior neural tube of 9.5 days post-coital embryos. At later developmental stages, a ventral-dorsal gradient of Hox 3.1 transcript accumulation is established. Hox 3.1 transcripts also are detected in the thoracic sclerotomes from the 6th to the 10th thoracic pre-vertebrae. The data support the hypothesis that the Hox 3.1 gene specifies positional information during murine embryogenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chromosome Mapping DNA/genetics Embryonic and Fetal Development Gene Expression Regulation Genes, Homeobox Mice Molecular Sequence Data Multigene Family Proteins/genetics
Chemicals
Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Breier G
Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, FRG.
Dressler G R
Gruss P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-05-00
Pages
1329-36
Language
English
Region
England
NLM ID
8208664
PMCID
PMC458379
Subset
IM
Databases
GENBANK
X07646, X07647
External Links
PubMed source PMC Full Text View journal details
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