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PMID: 2891029 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Murine Hox-1.7 homeo-box gene: cloning, chromosomal location, and expression.

Molecular and cellular biology ·Vol. 7 ·No. 10 ·1987-10-00 ·Pages 3836-41

Rubin MR, King W, Toth LE, Sawczuk IS, Levine MS, D'Eustachio P, Nguyen-Huu MC

Abstract

A new murine homeo-box, called Hox-1.7, has been identified in a rare cDNA from F9 teratocarcinoma stem cells. The Hox-1.7 homeo-box is 68 and 72% homologous to the Drosophila antennapedia (Antp) and iab-7 homeo-boxes, respectively. A major 2.5-kilobase transcript and several minor transcripts were detected by Northern blot (RNA blot) analysis in adult tissues as well as in midgestational embryos. The posterior spinal cord was found to be a major site of Hox-1.7 expression in 12.5-day-old embryos. Somatic cell hybrids were used to map the Hox-1.7 gene to mouse chromosome 6. Restriction fragment length polymorphisms associated with either the Hox-1.7 gene or the previously known Hox-1 complex were identified. Their distribution patterns in recombinant inbred mouse strains were used to determine the linkage between the two loci as well as to other loci on chromosome 6. This maps Hox-1 and Hox-1.7 close to two mouse loci that affect morphogenesis, postaxial hemimelia (px) and hypodactyly (Hd).

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chromosome Mapping Cloning, Molecular DNA/genetics Gene Expression Regulation Genes, Homeobox Mice/genetics Molecular Sequence Data Polymorphism, Restriction Fragment Length Species Specificity
Chemicals
DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rubin M R
Department of Microbiology, Columbia University, New York, New York 10032.
King W
Toth L E
Sawczuk I S
Levine M S
D'Eustachio P
Nguyen-Huu M C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.10.3836-3841.1987
Published
1987-10-00
Pages
3836-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368043
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/mcb.7.10.3836-3841.1987 PMC Full Text View journal details
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