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PMID: 6204862 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone.

The EMBO journal ·Vol. 3 ·No. 6 ·1984-06-00 ·Pages 1409-15

Rafalski JA, Scheets K, Metzler M, Peterson DM, Hedgcoth C, Söll DG

Abstract

Gliadins, the major wheat seed storage proteins, are encoded by a multigene family. Northern blot analysis shows that gliadin genes are transcribed in endosperm tissue into two classes of poly(A)+ mRNA, 1400 bases (class I) and 1600 bases (class II) in length. Using poly(A)+ RNA from developing wheat endosperm we constructed a cDNA library from which a number of clones coding for alpha/beta and gamma gliadins were identified by hybrid-selected mRNA translation and DNA sequencing. These cDNA clones were used as probes for the isolation of genomic gliadin clones from a wheat genomic library. One such genomic clone was characterized in detail and its DNA sequence determined. It contains a gene for a 33-kd alpha/beta gliadin protein (a 20 amino acid signal peptide and a 266 amino acid mature protein) which is very rich in glutamine (33.8%) and proline (15.4%). The gene sequence does not contain introns. A typical eukaryotic promoter sequence is present at -104 (relative to the translation initiation codon) and there are two normal polyadenylation signals 77 and 134 bases downstream from the translation termination codon. The coding sequence contains some internal sequence repetition, and is highly homologous to several alpha/beta gliadin cDNA clones. Homology to a gamma-gliadin cDNA clone is low, and there is no homology with known glutenin or zein cDNA sequences.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes Genes Gliadin/genetics Nucleic Acid Conformation Plant Development Plant Proteins/genetics Poly A/genetics Protein Biosynthesis RNA/genetics RNA, Messenger/genetics Transcription, Genetic Triticum/growth & development
Chemicals
Plant Proteins RNA, Messenger Poly A RNA DNA Gliadin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rafalski J A
Scheets K
Metzler M
Peterson D M
Hedgcoth C
Söll D G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-06-00
Pages
1409-15
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557531
Subset
IM
Databases
GENBANK
K03076
External Links
PubMed source PMC Full Text View journal details
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