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PMID: 6233138 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Primary structure of a genomic zein sequence of maize.

The EMBO journal ·Vol. 1 ·No. 11 ·1982-00-00 ·Pages 1337-42

Hu NT, Peifer MA, Heidecker G, Messing J, Rubenstein I

Abstract

The nucleotide sequence of a genomic clone (termed Z4 ) of the zein multigene family was compared to the nucleotide sequence of related cDNA clones of zein mRNAs. A tandem duplication of a 96-bp sequence is found in the genomic clone that is not present in the related cDNA clones. When the duplication is disregarded, the nucleotide sequence homology between Z4 and its related cDNAs was approximately 97%. The nucleotide sequence is also compared to other isolated cDNAs. No introns in the coding region of the zein gene are detected. The first nucleotide of a putative TATA box, TATAAATA , was located 88 nucleotides upstream of the first nucleotide of the first ATG codon which initiated the open reading frame. The first nucleotide of a putative CCAAT box, CAAAAT , appeared 45 nucleotides upstream of the first nucleotide of the zein cDNA clones in the 3' non-coding region also appeared in the genomic sequence at the same locations. The amino acid composition of the polypeptide specified by the Z4 nucleotide sequence is similar to the known composition of zein proteins.

MeSH Terms
Amino Acids/analysis Bacteriophage lambda Base Sequence Cloning, Molecular DNA/genetics Molecular Weight RNA, Messenger/genetics Zea mays/genetics Zein/genetics
Chemicals
Amino Acids RNA, Messenger DNA Zein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hu N T
Peifer M A
Heidecker G
Messing J
Rubenstein I
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1337-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553213
Subset
IM
Grants
NIGMS NIH HHS · GM24756 · United States
OHS HRSA HHS · ST32 GM07467-05 · United States
Databases
GENBANK
J01242, V01472, V01473, V01481
External Links
PubMed source PMC Full Text View journal details
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