Home LiteratureArticle Details
PMID: 6897917 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence analysis and comparison of cDNAs of the zein multigene family .

The EMBO journal ·Vol. 1 ·No. 11 ·1982-00-00 ·Pages 1329-35

Geraghty DE, Messing J, Rubenstein I

Abstract

The nucleotide sequence of two zein cDNAs in hybrid plasmids A20 and B49 have been determined. The insert in A20 is 921 bp long including a 5' non-coding region of 60 nucleotides, preceded by what is believed to be an artifactual sequence of 41 nucleotides, and a 3' non-coding region of 87 nucleotides. The B49 insert is 467 bp long and includes approximately one-half the protein coding sequence as well as a 3' non-coding region of 97 nucleotides. These sequences have been compared with the previously published sequence of another zein clone, A30 . A20 and A30 , both encoding 19 000 mol. wt. zeins , have approximately 85% homology at the nucleotide level. The B49 sequence, corresponding to a 22 000 mol. wt. zein, has approximately 65% homology to either A20 or A30 . All three zeins share common features including nearly identical amino acid compositions. In addition, the tandem repeats of 20 amino acids first seen in A30 are also present in A20 and B49 .

MeSH Terms
Amino Acid Sequence Base Sequence DNA/genetics Peptides/genetics Protein Sorting Signals Repetitive Sequences, Nucleic Acid Zea mays/genetics Zein/genetics
Chemicals
Peptides Protein Sorting Signals DNA Zein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Geraghty D E
Messing J
Rubenstein I
References (25)
25 references, click to expand
  1. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  2. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  3. Complex organization of zein genes in maize.
    Gene. 1981 Apr;13(3):239-49 PMID: 7262557
  4. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  5. Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localisation.
    EMBO J. 1982;1(1):53-8 PMID: 16453407
  6. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630-4 PMID: 6251474
  7. Synthesis and deposition of zein in protein bodies of maize endosperm.
    Plant Physiol. 1978 Aug;62(2):256-63 PMID: 16660496
  8. Primary structure of a genomic zein sequence of maize.
    EMBO J. 1982;1(11):1337-42 PMID: 6233138
  9. Genes and mRNAs coding for zein polypeptides in Zea mays.
    Eur J Biochem. 1979 Dec;102(1):211-22 PMID: 520323
  10. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  11. Clustering of genes for 20 kd zein subunits in the short arm of maize chromosome 7.
    Genetics. 1981 Feb;97(2):363-77 PMID: 17249075
  12. Heterogeneity of zein mRNA and protein in maize.
    Plant Physiol. 1980 Jan;65(1):98-106 PMID: 16661153
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. Translocation of proteins across membranes: the signal hypothesis and beyond.
    Symp Soc Exp Biol. 1979;33:9-36 PMID: 524275
  15. The collagen gene: evidence for its evolutinary assembly by amplification of a DNA segment containing an exon of 54 bp.
    Cell. 1980 Dec;22(3):887-92 PMID: 7460017
  16. Primary sequence of the 5' flanking regions of the Drosophila heat shock genes in chromosome subdivision 67B.
    Nucleic Acids Res. 1981 Apr 10;9(7):1627-42 PMID: 6262735
  17. Apple II software for M13 shotgun DNA sequencing.
    Nucleic Acids Res. 1982 Jan 11;10(1):39-49 PMID: 6278410
  18. The primary structure of a plant storage protein: zein.
    Nucleic Acids Res. 1981 Oct 10;9(19):5163-74 PMID: 6895552
  19. Purification and translation of zein messenger RNA from maize endosperm protein bodies.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):696-700 PMID: 16592496
  20. The primary structures of two leghemoglobin genes from soybean.
    Nucleic Acids Res. 1982 Jan 22;10(2):689-701 PMID: 6278428
  21. Introduction by molecular cloning of artifactual inverted sequences at the 5' terminus of the sense strand of bovine parathyroid hormone cDNA.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4073-7 PMID: 6170060
  22. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  23. Secretion and membrane localization of proteins in Escherichia coli.
    CRC Crit Rev Biochem. 1980;7(4):339-71 PMID: 6993100
  24. Codon catalog usage is a genome strategy modulated for gene expressivity.
    Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
  25. Analysis of the complexity and frequency of zein genes in the maize genome.
    Biochemistry. 1980 Apr 15;19(8):1644-50 PMID: 6155140
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1329-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553212
Subset
IM
Grants
NIGMS NIH HHS · GM07094 · United States
NIGMS NIH HHS · GM24756 · United States
Databases
GENBANK
J01242, V01476, V01477, V01481
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2