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PMID: 6262735 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Primary sequence of the 5' flanking regions of the Drosophila heat shock genes in chromosome subdivision 67B.

Nucleic acids research ·Vol. 9 ·No. 7 ·1981-04-10 ·Pages 1627-42

Ingolia TD, Craig EA

Abstract

The 5' flanking regions of the four small heat shock genes of Drosophila melanogaster from cytological locus 67B have been characterized. Approximately 500 bp of the primary sequence upstream from the proposed site of initiation of translation has been determined and the 5' end of the messenger RNAs have been localized for each gene. Each of the four genes contains an A-T rich sequence, either TATAAATA or TATAAAAG, which is flanked by a G-C rich region. This A-T rich sequence, which ends about 23 bp upstream from the proposed site of initiation of transcription, is similar to those found in most eukaryotic genes. Novel features of these four genes include a region of homology beginning near the proposed site of initiation of transcription and extending about 20 bp into the 5' noncoding region of the genes. This sequence is also found in the gene for the major heat shock protein, hsp 70. The leaders of these five heat shock genes are long, from 111 to 253 bases in length, as well as unusually A rich, from 46% to 51% A. In addition, each of the four small genes contains the sequence ACTTTNA, 195 +/- 12 bp from the proposed site of initiation of transcription.

MeSH Terms
Animals Base Sequence Chromosomes/analysis DNA/genetics DNA Restriction Enzymes Drosophila melanogaster/genetics Genes Hot Temperature RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ingolia T D
Craig E A
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.7.1627
Published
1981-04-10
Pages
1627-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326787
Subset
IM
Databases
GENBANK
J01090, J01091, J01092, J01093, J01094, J01095, J01096, J01097, J01098, J01099, J01100, J01101
External Links
PubMed source DOI: 10.1093/nar/9.7.1627 PMC Full Text View journal details
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