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PMID: 6253919 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Four Drosophila heat shock genes at 67B: characterization of recombinant plasmids.

Nucleic acids research ·Vol. 8 ·No. 19 ·1980-10-10 ·Pages 4441-57

Craig EA, McCarthy BJ

Abstract

A 33 kilobase region of the 67B locus of Drosophila melanogaster genome has been isolated. The genes for the 27K, 26K, 23K and 22K heat shock induced proteins are contained within an 11 kb segment. the 27K gene, approximately 1.4 kb in length, and the 23K gene, approximately 1.0 kb in length, are separated by about 1.5 kb of spacer DNA; a third block of transcription, which encodes the 26K gene, is approximately 1.1 kb in length, and is separated from the gene for the 23K protein by 4.5 kb of spacer DNA. The 22K gene is located approximately 1.2 kb from the 26K gene. The RNAs encoding the 27K, 23K and 22K genes are transcribed from the same DNA strand. With the assignment of the four small heat shock induced proteins to 67B, genes for all seven major heat shock induced proteins have no been mapped cytologically.

MeSH Terms
Animals Chromosomes/analysis DNA Restriction Enzymes DNA, Recombinant/metabolism Drosophila melanogaster/metabolism Genes Heat-Shock Proteins Molecular Weight Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Messenger/biosynthesis Transcription, Genetic
Chemicals
DNA, Recombinant Heat-Shock Proteins RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Craig E A
McCarthy B J
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.19.4441
Published
1980-10-10
Pages
4441-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324250
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.19.4441 PMC Full Text View journal details
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