Home LiteratureArticle Details
PMID: 6269062 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing.

Nucleic acids research ·Vol. 9 ·No. 12 ·1981-06-25 ·Pages 2871-88

Gardner RC, Howarth AJ, Hahn P, Brown-Luedi M, Shepherd RJ, Messing J

Abstract

We have determined the complete primary structure (8031 base pairs) of an infectious clone of cauliflower mosaic virus strain CM1841. The sequence was obtained using the strategy of cloning shotgun restriction fragments in the sequencing vector M13mp7. Comparison of the CM1841 sequence with that published for another caMV strain (Strasbourg) reveals 4.4% changes, mostly nucleotide substitutions with a few small insertions and deletions. The six open reading frames in the sequence of the Strasbourg isolate are also present in CM1841.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Circular/genetics DNA, Viral/genetics Escherichia coli/genetics Mosaic Viruses/genetics Mutation Plasmids
Chemicals
DNA, Circular DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gardner R C
Howarth A J
Hahn P
Brown-Luedi M
Shepherd R J
Messing J
References (25)
25 references, click to expand
  1. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  2. Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7024-8 PMID: 6938951
  3. The structure of cauliflower mosaic virus. I. A restriction endonuclease map of cauliflower mosaic virus DNA.
    Virology. 1977 Jul 15;80(2):362-75 PMID: 329557
  4. Isolation and Mapping of Small Cauliflower Mosaic Virus DNA Fragments Active as Promoters in Escherichia coli.
    J Virol. 1981 Feb;37(2):673-82 PMID: 16789206
  5. Replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplasts.
    Virology. 1978 May 15;86(2):468-81 PMID: 664242
  6. Studies on the single-stranded discontinuities of the cauliflower mosaic virus genome.
    Nucleic Acids Res. 1978 Aug;5(8):2913-25 PMID: 693323
  7. BK virus DNA: complete nucleotide sequence of a human tumor virus.
    Science. 1979 Oct 26;206(4417):456-62 PMID: 228391
  8. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  9. Restriction map of native and cloned cauliflower mosaic virus DNA.
    Gene. 1980 Oct;11(1-2):21-31 PMID: 7002732
  10. Second EcoRI fragment of F capable of self-replication.
    J Bacteriol. 1979 Jul;139(1):141-51 PMID: 378951
  11. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.
    Nature. 1978 Mar 23;272(5651):375-7 PMID: 416358
  12. The polarity of the cauliflower mosaic virus genome.
    Nucleic Acids Res. 1979 Oct 10;7(3):669-77 PMID: 503842
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  15. Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6 PMID: 6938957
  16. Nucleotide sequence of cauliflower mosaic virus DNA.
    Cell. 1980 Aug;21(1):285-94 PMID: 7407912
  17. Nucleotide sequence of bacteriophage G4 DNA.
    Nature. 1978 Nov 16;276(5685):236-47 PMID: 714153
  18. A restriction map of cauliflower mosaic virus DNA (strain PV 147). Mapping of the cleavage sites of HhaI, SacI, AvaI, PvuII, PstI, XbaI, EcoRI, Bg/II, HincII, HpaII and HindII + III.
    Eur J Biochem. 1979 Oct;100(1):245-55 PMID: 488094
  19. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  20. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.
    Gene. 1980 Oct;11(1-2):129-48 PMID: 6254849
  21. Structure of the cauliflower mosaic virus genome. II. Variation in DNA structure and sequence between isolates.
    Virology. 1978 May 15;86(2):482-93 PMID: 664243
  22. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  23. Computer analysis of nucleic acid regulatory sequences.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4401-5 PMID: 270683
  24. The nucleotide sequence of an untranslated but conserved domain at the 3' end of the avian sarcoma virus genome.
    Nucleic Acids Res. 1980 Jul 11;8(13):2967-84 PMID: 6253899
  25. The rapid purification of T4 DNA ligase from a lambda T4 lig lysogen.
    J Biol Chem. 1980 Feb 10;255(3):813-5 PMID: 6985906
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.12.2871
Published
1981-06-25
Pages
2871-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326899
Subset
IM
Databases
GENBANK
J02046
External Links
PubMed source DOI: 10.1093/nar/9.12.2871 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2