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PMID: 6174944 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and analysis of cDNA sequences derived from poly A+ RNA from barley endosperm: identification of B hordein related clones.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 6689-707

Forde BG, Kreis M, Bahramian MB, Matthews JA, Miflin BJ, Thompson RD, Bartels D, Flavell RB

Abstract

A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Recombinant Glutens Hordeum/genetics Plant Proteins/genetics Plants/genetics Poly A/genetics RNA/genetics RNA, Messenger Seeds
Chemicals
DNA, Recombinant Plant Proteins RNA, Messenger Poly A RNA Glutens
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Forde B G
Kreis M
Bahramian M B
Matthews J A
Miflin B J
Thompson R D
Bartels D
Flavell R B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.24.6689
Published
1981-12-21
Pages
6689-707
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327634
Subset
IM
Databases
GENBANK
J01237
External Links
PubMed source DOI: 10.1093/nar/9.24.6689 PMC Full Text View journal details
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